Title |
YB-1 Relocates to the Nucleus in Adenovirus-infected Cells and Facilitates Viral Replication by Inducing E2 Gene Expression through the E2 Late Promoter*
|
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Published in |
Journal of Biological Chemistry, January 2002
|
DOI | 10.1074/jbc.m106955200 |
Pubmed ID | |
Authors |
Per S. Holm, Stephan Bergmann, Karsten Jürchott, Hermann Lage, Karsten Brand, Axel Ladhoff, Klaus Mantwill, David T. Curiel, Matthias Dobbelstein, Manfred Dietel, Bernd Gänsbacher, Hans-Dieter Royer |
Abstract |
The adenovirus early proteins E1A and E1B-55kDa are key regulators of viral DNA replication, and it was thought that targeting of p53 by E1B-55kDa is essential for this process. Here we have identified a previously unrecognized function of E1B for adenovirus replication. We found that E1B-55kDa is involved in targeting the transcription factor YB-1 to the nuclei of adenovirus type 5-infected cells where it is associated with viral inclusion bodies believed to be sites of viral transcription and replication. We show that YB-1 facilitates E2 gene expression through the E2 late promoter thus controlling E2 gene activity at later stages of infection. The role of YB-1 for adenovirus replication was demonstrated with an E1-minus adenovirus vector containing a YB-1 transgene. In infected cells, AdYB-1 efficiently replicated and produced infectious progeny particles. Thus, adenovirus E1B-55kDa protein and the host cell factor YB-1 act jointly to facilitate adenovirus replication in the late phase of infection. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 2% |
Unknown | 58 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 15 | 25% |
Student > Ph. D. Student | 10 | 17% |
Student > Master | 9 | 15% |
Student > Bachelor | 7 | 12% |
Student > Doctoral Student | 2 | 3% |
Other | 7 | 12% |
Unknown | 9 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 22 | 37% |
Biochemistry, Genetics and Molecular Biology | 19 | 32% |
Medicine and Dentistry | 4 | 7% |
Linguistics | 1 | 2% |
Immunology and Microbiology | 1 | 2% |
Other | 3 | 5% |
Unknown | 9 | 15% |