| Title |
Quantification and profiling of lipophilic marine toxins in microalgae by UHPLC coupled to high-resolution orbitrap mass spectrometry
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, April 2015
|
| DOI | 10.1007/s00216-015-8637-y |
| Pubmed ID | |
| Authors |
Gabriel Orellana, Lieven Van Meulebroek, Sarah Van Vooren, Maarten De Rijcke, Michiel Vandegehuchte, Colin R. Janssen, Lynn Vanhaecke |
| Abstract |
During the last decade, a significant increase in the occurrence of harmful algal blooms (HABs), linked to repetitive cases of shellfish contamination has become a public health concern and therefore, accurate methods to detect marine toxins in different matrices are required. In this study, we developed a method for profiling lipophilic marine microalgal toxins based on ultra-high-performance liquid chromatography coupled to high-resolution Orbitrap mass spectrometry (UHPLC-HR-Orbitrap MS). Extraction of selected toxins (okadaic acid (OA), dinophysistoxin-1 (DTX-1), pectenotoxin-2 (PTX-2), azaspiracid-1 (AZA-1), yessotoxin (YTX) and 13-desmethyl spirolide C (SPX-1)) was optimized using a Plackett-Burman design. Three key algal species, i.e., Prorocentrum lima, Protoceratium reticulatum and Alexandrium ostenfeldii were used to test the extraction efficiency of OA, YTXs and SPXs, respectively. Prorocentrum micans, fortified with certified reference solutions, was used for recovery studies. The quantitative and confirmatory performance of the method was evaluated according to CD 2002/657/EC. Limits of detection and quantification ranged between 0.006 and 0.050 ng mL(-1) and 0.018 to 0.227 ng mL(-1), respectively. The intra-laboratory reproducibility ranged from 6.8 to 11.7 %, repeatability from 6.41 to 11.5 % and mean corrected recoveries from 81.9 to 119.6 %. In addition, algae cultures were retrospectively screened for analogues and metabolites through a homemade database. Using the ToxID software programme, 18 toxin derivates were detected in the extract of three toxin producing microalgae species. In conclusion, the generic extraction and full-scan HRMS approach offers an excellent quantitative performance and simultaneously allows to profile analogues and metabolites of marine toxins in microalgae. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Belgium | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 67% |
| Members of the public | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Chile | 1 | 2% |
| United States | 1 | 2% |
| Norway | 1 | 2% |
| Canada | 1 | 2% |
| Unknown | 37 | 90% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 11 | 27% |
| Student > Master | 9 | 22% |
| Student > Ph. D. Student | 6 | 15% |
| Student > Doctoral Student | 3 | 7% |
| Lecturer | 2 | 5% |
| Other | 5 | 12% |
| Unknown | 5 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 14 | 34% |
| Environmental Science | 5 | 12% |
| Agricultural and Biological Sciences | 5 | 12% |
| Biochemistry, Genetics and Molecular Biology | 4 | 10% |
| Engineering | 2 | 5% |
| Other | 4 | 10% |
| Unknown | 7 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 August 2015.
All research outputs
#15,890,477
of 25,604,262 outputs
Outputs from Analytical & Bioanalytical Chemistry
#4,787
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Outputs of similar age
#146,766
of 280,428 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#42
of 180 outputs
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