↓ Skip to main content

Thioredoxin Expression and Localization in Human Cell Lines: Detection of Full-Length and Truncated Species

Overview of attention for article published in Experimental Cell Research, October 1997
Altmetric Badge

Mentioned by

patent
1 patent

Citations

dimensions_citation
75 Dimensions

Readers on

mendeley
21 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Thioredoxin Expression and Localization in Human Cell Lines: Detection of Full-Length and Truncated Species
Published in
Experimental Cell Research, October 1997
DOI 10.1006/excr.1997.3699
Pubmed ID
Authors

Bita Sahaf, Anita Söderberg, Giannis Spyrou, Ana Maria Barral, Klas Pekkari, Arne Holmgren, Anders Rosén

Abstract

Thioredoxin (Trx) is an intracellular multifunctional 12-kDa protein with a reduction/oxidation (redox) active disulfide constitutively expressed by most cells of the human body. Trx can also be released by cells such as lymphocytes upon activation or oxidative stress exposure and exert a cocytokine and cytoprotective activity. In addition, a truncated 10-kDa form of Trx has been reported. In order to better understand the function of full-length and truncated Trx, we have produced, for the first time, specific monoclonal antibodies, which can discriminate between the two forms. Using these novel antibodies, designated alpha Trx1 to alpha Trx4, a panel of cell lines derived from human B and T lymphocytes, monocytes, granulocytes, and melanomas was analyzed by immunochemical techniques. The cellular distribution differed between the two forms. All lines contained full-length Trx, also located to a minor extent on the cell surface. One exception was the melanoma cell line FM28.4, which did not show any Trx expression. Truncated Trx was present in most cells in minimal amounts only, whereas the monocytic cell lines THP-1 and U-937 expressed high amounts on the cell surface, as shown by flow cytometric analysis of living cells and confocal laser-scanning microscopy. The biological importance and function of the short versus long forms of Trx as detected by the antibodies are discussed.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 10%
Czechia 1 5%
France 1 5%
Unknown 17 81%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 29%
Student > Ph. D. Student 5 24%
Student > Bachelor 3 14%
Student > Doctoral Student 1 5%
Professor 1 5%
Other 2 10%
Unknown 3 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 29%
Agricultural and Biological Sciences 6 29%
Pharmacology, Toxicology and Pharmaceutical Science 1 5%
Immunology and Microbiology 1 5%
Medicine and Dentistry 1 5%
Other 1 5%
Unknown 5 24%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 August 2013.
All research outputs
#8,535,472
of 25,374,647 outputs
Outputs from Experimental Cell Research
#1,345
of 5,318 outputs
Outputs of similar age
#9,366
of 28,974 outputs
Outputs of similar age from Experimental Cell Research
#6
of 22 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,318 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 31st percentile – i.e., 31% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 28,974 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 9th percentile – i.e., 9% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 22 others from the same source and published within six weeks on either side of this one. This one is in the 4th percentile – i.e., 4% of its contemporaries scored the same or lower than it.