Title |
Distribution and phylogenetic analysis of Culex flavivirus in mosquitoes in China
|
---|---|
Published in |
Archives of Virology, June 2015
|
DOI | 10.1007/s00705-015-2492-1 |
Pubmed ID | |
Authors |
Wenkai Liang, Xiaoxia He, Guifang Liu, Shaobai Zhang, Shihong Fu, Min Wang, Weixin Chen, Ying He, Xiaoyan Tao, Hongyue Jiang, Xiaojuan Lin, Xiaoyan Gao, Weijun Hu, Yan Liu, Lei Feng, Yuxi Cao, Guang Yang, Chunxia Jing, Guodong Liang, Huanyu Wang |
Abstract |
Culex flavivirus (CxFV) is an insect-specific virus of the genus Flavivirus. CxFV strains have been isolated from Cx. pipiens, Cx. quinquefasciatus, and other Cx. species in Asia, Africa, North America, Central America and South America. CxFV was isolated for the first time in China in 2006. As this is a novel flavivirus, we explored the distribution and genetic characteristics of Culex flavivirus in China. A total of 46,649 mosquitoes were collected in seven provinces between 2004 and 2012 and were analysed in 871 pools. 29 CxFV RNAs from Cx. pipiens, Cx. tritaeniorhynchus, Anopheles Sinensis, and Culex spp. tested positive for CxFV in real-time RT-PCR. 6 CxFV strains were isolated from Cx. species collected in Shandong, Henan, and Shaanxi provinces, while no virus or viral RNA was detected in samples from Sichuan, Chongqing, Hubei, and Fujian. Phylogenetic analysis of the envelope gene indicated that Chinese strains formed a robust subgroup of genotype 1, together with viruses from the United States and Japan. This study demonstrates that the geographic distribution of CxFV in China is widespread, but geographical boundaries to spread are apparent. Our findings suggest that CxFV can infect various mosquito species in nature. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 26 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 4 | 15% |
Student > Master | 3 | 12% |
Researcher | 2 | 8% |
Student > Postgraduate | 2 | 8% |
Student > Doctoral Student | 1 | 4% |
Other | 4 | 15% |
Unknown | 10 | 38% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 5 | 19% |
Biochemistry, Genetics and Molecular Biology | 4 | 15% |
Veterinary Science and Veterinary Medicine | 2 | 8% |
Medicine and Dentistry | 2 | 8% |
Materials Science | 1 | 4% |
Other | 0 | 0% |
Unknown | 12 | 46% |