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Mendeley readers
Attention Score in Context
| Title |
Multi-wavelength immunoassays using surface plasmon-coupled emission
|
|---|---|
| Published in |
Biochemical & Biophysical Research Communications, January 2004
|
| DOI | 10.1016/j.bbrc.2003.12.010 |
| Pubmed ID | |
| Authors |
Evgenia Matveeva, Joanna Malicka, Ignacy Gryczynski, Zygmunt Gryczynski, Joseph R. Lakowicz |
| Abstract |
We describe a new method for multi-wavelength immunoassays using surface plasmon-coupled emission (SPCE). This phenomenon is coupling of excited fluorophores with a nearby thin metal film, in our case silver, resulting in strongly directional emission into the underlying glass substrate. The angle at which the radiation propagate through the prism depends on the surface plasmon angle for the relevant wavelength. These angles depend on emission wavelength, allowing measurement of multiple analytes using multiple emission wavelengths. We demonstrated this possibility using antibodies labeled with either Rhodamine Red-X or AlexaFluor 647. These antibodies were directed against an antigen protein bound to the silver surface. The emission from each labeled antibody occurred at a different angle on the glass prism, allowing independent measurement of surface binding of each antibody. This method of SPCE immunoassays can be readily extended to 4 or more wavelengths. |
Mendeley readers
The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 4% |
| Unknown | 22 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 39% |
| Researcher | 6 | 26% |
| Student > Master | 4 | 17% |
| Professor > Associate Professor | 2 | 9% |
| Lecturer > Senior Lecturer | 1 | 4% |
| Other | 1 | 4% |
| Readers by discipline | Count | As % |
|---|---|---|
| Physics and Astronomy | 11 | 48% |
| Engineering | 3 | 13% |
| Agricultural and Biological Sciences | 2 | 9% |
| Chemistry | 2 | 9% |
| Materials Science | 2 | 9% |
| Other | 3 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 November 2017.
All research outputs
#8,586,083
of 25,498,750 outputs
Outputs from Biochemical & Biophysical Research Communications
#7,645
of 26,678 outputs
Outputs of similar age
#36,649
of 144,157 outputs
Outputs of similar age from Biochemical & Biophysical Research Communications
#83
of 222 outputs
Altmetric has tracked 25,498,750 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 26,678 research outputs from this source. They receive a mean Attention Score of 4.5. This one is in the 28th percentile – i.e., 28% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 144,157 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 222 others from the same source and published within six weeks on either side of this one. This one is in the 2nd percentile – i.e., 2% of its contemporaries scored the same or lower than it.