| Title |
Artifacts in single-molecule localization microscopy
|
|---|---|
| Published in |
Histochemistry and Cell Biology, July 2015
|
| DOI | 10.1007/s00418-015-1340-4 |
| Pubmed ID | |
| Authors |
Anne Burgert, Sebastian Letschert, Sören Doose, Markus Sauer |
| Abstract |
Single-molecule localization microscopy provides subdiffraction resolution images with virtually molecular resolution. Through the availability of commercial instruments and open-source reconstruction software, achieving super resolution is now public domain. However, despite its conceptual simplicity, localization microscopy remains prone to user errors. Using direct stochastic optical reconstruction microscopy, we investigate the impact of irradiation intensity, label density and photoswitching behavior on the distribution of membrane proteins in reconstructed super-resolution images. We demonstrate that high emitter densities in combination with inappropriate photoswitching rates give rise to the appearance of artificial membrane clusters. Especially, two-dimensional imaging of intrinsically three-dimensional membrane structures like microvilli, filopodia, overlapping membranes and vesicles with high local emitter densities is prone to generate artifacts. To judge the quality and reliability of super-resolution images, the single-molecule movies recorded to reconstruct the images have to be carefully investigated especially when investigating membrane organization and cluster analysis. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 117 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | <1% |
| Netherlands | 1 | <1% |
| France | 1 | <1% |
| Ireland | 1 | <1% |
| Czechia | 1 | <1% |
| United Kingdom | 1 | <1% |
| Unknown | 111 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 40 | 34% |
| Researcher | 23 | 20% |
| Student > Master | 11 | 9% |
| Student > Doctoral Student | 9 | 8% |
| Other | 5 | 4% |
| Other | 11 | 9% |
| Unknown | 18 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 21 | 18% |
| Physics and Astronomy | 21 | 18% |
| Biochemistry, Genetics and Molecular Biology | 18 | 15% |
| Chemistry | 11 | 9% |
| Engineering | 9 | 8% |
| Other | 16 | 14% |
| Unknown | 21 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 November 2021.
All research outputs
#7,347,192
of 24,217,893 outputs
Outputs from Histochemistry and Cell Biology
#214
of 926 outputs
Outputs of similar age
#80,758
of 267,115 outputs
Outputs of similar age from Histochemistry and Cell Biology
#3
of 14 outputs
Altmetric has tracked 24,217,893 research outputs across all sources so far. This one has received more attention than most of these and is in the 69th percentile.
So far Altmetric has tracked 926 research outputs from this source. They receive a mean Attention Score of 3.6. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 267,115 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.
We're also able to compare this research output to 14 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 78% of its contemporaries.