| Title |
Fluorometric aptamer based assay for ochratoxin A based on the use of exonuclease III
|
|---|---|
| Published in |
Microchimica Acta, April 2018
|
| DOI | 10.1007/s00604-018-2786-6 |
| Pubmed ID | |
| Authors |
Renjie Liu, Hua Wu, Lei Lv, Xiaojiao Kang, Chengbi Cui, Jin Feng, Zhijun Guo |
| Abstract |
This study describes an aptamer based assay for the mycotoxin ochratoxin A (OTA). The method is based on the use of an OTA-specific aptamer, exonuclease (Exo) III, SYBR Gold as a fluorescent probe, and a complementary strand that specifically combines with the aptamer. In the presence of OTA, the aptamer and OTA hybridize, thereby resulting in the formation of ssDNA, which is not digested by Exo III. Intense fluorescence is observed after addition of SYBR Gold (best measured at excitation/emission wavelengths of 495/540 nm). Fluorescence increases linearly with the log of the OTA concentration in the range from 8 to 1000 ng·mL-1. The detection limit is 4.7 ng·mL-1. The assay was applied to the determination of OTA in diluted [2%(v/v)] red wine, and recoveries and RSDs ranged between 93.5% and 113.8%, and between 3.2% and 5.7%, respectively. Graphical abstract In the presence of ochratoxin A (OTA), specific combinations of aptamer and OTA may occur and result in DNA double strands being untied, which avoids being digested by Exo III. Intense fluorescence is observed after SYBR Gold addition. |
Mendeley readers
The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 19 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 4 | 21% |
| Researcher | 3 | 16% |
| Student > Ph. D. Student | 2 | 11% |
| Unspecified | 2 | 11% |
| Professor > Associate Professor | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 6 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 3 | 16% |
| Biochemistry, Genetics and Molecular Biology | 3 | 16% |
| Unspecified | 2 | 11% |
| Arts and Humanities | 1 | 5% |
| Economics, Econometrics and Finance | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 8 | 42% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 May 2018.
All research outputs
#20,504,518
of 23,070,218 outputs
Outputs from Microchimica Acta
#1,039
of 1,402 outputs
Outputs of similar age
#288,999
of 327,748 outputs
Outputs of similar age from Microchimica Acta
#13
of 27 outputs
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