| Title |
Precise and economic FIB/SEM for CLEM: with 2 nm voxels through mitosis
|
|---|---|
| Published in |
Histochemistry and Cell Biology, May 2018
|
| DOI | 10.1007/s00418-018-1681-x |
| Pubmed ID | |
| Authors |
Manja Luckner, Gerhard Wanner |
| Abstract |
A portfolio is presented documenting economic, high-resolution correlative focused ion beam scanning electron microscopy (FIB/SEM) in routine, comprising: (i) the use of custom-labeled slides and coverslips, (ii) embedding of cells in thin, or ultra-thin resin layers for correlative light and electron microscopy (CLEM) and (iii) the claim to reach the highest resolution possible with FIB/SEM in xyz. Regions of interest (ROIs) defined in light microscope (LM), can be relocated quickly and precisely in SEM. As proof of principle, HeLa cells were investigated in 3D context at all stages of the cell cycle, documenting ultrastructural changes during mitosis: nuclear envelope breakdown and reassembly, Golgi degradation and reconstitution and the formation of the midzone and midbody. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 37 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 10 | 27% |
| Student > Ph. D. Student | 8 | 22% |
| Student > Bachelor | 4 | 11% |
| Student > Doctoral Student | 2 | 5% |
| Other | 2 | 5% |
| Other | 6 | 16% |
| Unknown | 5 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 24% |
| Biochemistry, Genetics and Molecular Biology | 8 | 22% |
| Computer Science | 3 | 8% |
| Medicine and Dentistry | 3 | 8% |
| Engineering | 2 | 5% |
| Other | 5 | 14% |
| Unknown | 7 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 December 2021.
All research outputs
#3,359,878
of 24,217,893 outputs
Outputs from Histochemistry and Cell Biology
#44
of 926 outputs
Outputs of similar age
#66,193
of 334,146 outputs
Outputs of similar age from Histochemistry and Cell Biology
#2
of 18 outputs
Altmetric has tracked 24,217,893 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 926 research outputs from this source. They receive a mean Attention Score of 3.6. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 334,146 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 88% of its contemporaries.