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Assessment of Genetic Markers for Tracking the Sources of Human Wastewater Associated Escherichia coli in Environmental Waters

Overview of attention for article published in Environmental Science & Technology, July 2015
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Title
Assessment of Genetic Markers for Tracking the Sources of Human Wastewater Associated Escherichia coli in Environmental Waters
Published in
Environmental Science & Technology, July 2015
DOI 10.1021/acs.est.5b02163
Pubmed ID
Authors

Ahmed Warish, Cheryl Triplett, Ryota Gomi, Pradip Gyawali, Leonie Hodgers, Simon Toze

Abstract

In this study, we have evaluated the performance characteristics (host-specificity and -sensitivity) of four human wastewater-associated Escherichia coli (E. coli) genetic markers (H8, H12, H14 and H24) in ten target (human) and non-target (cat, cattle, deer, dog, emu, goat, horse, kangaroo and possum) host groups in Southeast Queensland, Australia. The overall host-sensitivity values of the tested markers in human wastewater samples were 1.0. The overall host-specificity values of these markers to differentiate between human and animal host groups were 0.94, 0.85, 0.72, and 0.57 for H8, H12, H24 and H14, respectively. Based on the higher host-specificity values, H8 and H12 markers were chosen for a validation environmental study. The prevalence of the H8 and H12 markers was determined among human wastewater E. coli isolates collected from a wastewater treatment plant (WWTP). Among the 97 isolates tested, 44 (45%) and 14 (14%) were positive for the H8 and H12 markers, respectively. A total of 307 E. coli isolates were tested from environmental water samples collected in Brisbane, of which 7% and 20% were also positive for the H8 and H12 markers, respectively. Based on our results, we recommend that these markers could be useful when it is important to identify the source(s) of E. coli (whether they originated from human wastewater or not) in environmental waters.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 48 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 2%
Unknown 47 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 11 23%
Student > Ph. D. Student 8 17%
Student > Master 8 17%
Student > Bachelor 5 10%
Other 4 8%
Other 2 4%
Unknown 10 21%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 25%
Environmental Science 11 23%
Biochemistry, Genetics and Molecular Biology 4 8%
Engineering 3 6%
Immunology and Microbiology 2 4%
Other 3 6%
Unknown 13 27%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 July 2015.
All research outputs
#22,760,732
of 25,377,790 outputs
Outputs from Environmental Science & Technology
#19,590
of 20,675 outputs
Outputs of similar age
#235,188
of 275,281 outputs
Outputs of similar age from Environmental Science & Technology
#263
of 288 outputs
Altmetric has tracked 25,377,790 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 20,675 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 17.8. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 288 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.