Title |
Quantitative Assessment of Protein Structural Models by Comparison of H/D Exchange MS Data with Exchange Behavior Accurately Predicted by DXCOREX
|
---|---|
Published in |
Journal of the American Society for Mass Spectrometry, October 2011
|
DOI | 10.1007/s13361-011-0267-9 |
Pubmed ID | |
Authors |
Tong Liu, Dennis Pantazatos, Sheng Li, Yoshitomo Hamuro, Vincent J. Hilser, Virgil L. Woods |
Abstract |
Peptide amide hydrogen/deuterium exchange mass spectrometry (DXMS) data are often used to qualitatively support models for protein structure. We have developed and validated a method (DXCOREX) by which exchange data can be used to quantitatively assess the accuracy of three-dimensional (3-D) models of protein structure. The method utilizes the COREX algorithm to predict a protein's amide hydrogen exchange rates by reference to a hypothesized structure, and these values are used to generate a virtual data set (deuteron incorporation per peptide) that can be quantitatively compared with the deuteration level of the peptide probes measured by hydrogen exchange experimentation. The accuracy of DXCOREX was established in studies performed with 13 proteins for which both high-resolution structures and experimental data were available. The DXCOREX-calculated and experimental data for each protein was highly correlated. We then employed correlation analysis of DXCOREX-calculated versus DXMS experimental data to assess the accuracy of a recently proposed structural model for the catalytic domain of a Ca(2+)-independent phospholipase A(2). The model's calculated exchange behavior was highly correlated with the experimental exchange results available for the protein, supporting the accuracy of the proposed model. This method of analysis will substantially increase the precision with which experimental hydrogen exchange data can help decipher challenging questions regarding protein structure and dynamics. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 4 | 6% |
India | 1 | 2% |
Argentina | 1 | 2% |
Unknown | 59 | 91% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 22 | 34% |
Researcher | 17 | 26% |
Professor > Associate Professor | 5 | 8% |
Student > Master | 5 | 8% |
Student > Bachelor | 4 | 6% |
Other | 7 | 11% |
Unknown | 5 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 17 | 26% |
Agricultural and Biological Sciences | 15 | 23% |
Chemistry | 14 | 22% |
Computer Science | 2 | 3% |
Medicine and Dentistry | 2 | 3% |
Other | 8 | 12% |
Unknown | 7 | 11% |