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Comparative genome analysis identifies novel nucleic acid diagnostic targets for use in the specific detection of Haemophilus influenzae

Overview of attention for article published in Diagnostic Microbiology & Infectious Disease, June 2015
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  • Above-average Attention Score compared to outputs of the same age and source (59th percentile)

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Title
Comparative genome analysis identifies novel nucleic acid diagnostic targets for use in the specific detection of Haemophilus influenzae
Published in
Diagnostic Microbiology & Infectious Disease, June 2015
DOI 10.1016/j.diagmicrobio.2015.06.013
Pubmed ID
Authors

Helena Coughlan, Kate Reddington, Nina Tuite, Teck Wee Boo, Martin Cormican, Louise Barrett, Terry J Smith, Eoin Clancy, Thomas Barry

Abstract

Haemophilus influenzae is recognised as an important human pathogen associated with invasive infections, including bloodstream infection and meningitis. Currently used molecular-based diagnostic assays lack specificity in correctly detecting and identifying H. influenzae. As such, there is a need to develop novel diagnostic assays for the specific identification of H. influenzae. Whole genome comparative analysis was performed to identify putative diagnostic targets, which are unique in nucleotide sequence to H. influenzae. From this analysis, we identified 2H. influenzae putative diagnostic targets, phoB and pstA, for use in real-time PCR diagnostic assays. Real-time PCR diagnostic assays using these targets were designed and optimised to specifically detect and identify all 55H. influenzae strains tested. These novel rapid assays can be applied to the specific detection and identification of H. influenzae for use in epidemiological studies and could also enable improved monitoring of invasive disease caused by these bacteria.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Colombia 1 5%
Unknown 19 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 25%
Student > Bachelor 3 15%
Researcher 3 15%
Student > Master 3 15%
Professor > Associate Professor 2 10%
Other 2 10%
Unknown 2 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 40%
Biochemistry, Genetics and Molecular Biology 3 15%
Medicine and Dentistry 3 15%
Immunology and Microbiology 3 15%
Environmental Science 1 5%
Other 0 0%
Unknown 2 10%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 July 2015.
All research outputs
#16,045,990
of 25,371,288 outputs
Outputs from Diagnostic Microbiology & Infectious Disease
#1,345
of 2,303 outputs
Outputs of similar age
#146,606
of 278,286 outputs
Outputs of similar age from Diagnostic Microbiology & Infectious Disease
#15
of 44 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. This one is in the 34th percentile – i.e., 34% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,303 research outputs from this source. They receive a mean Attention Score of 3.9. This one is in the 39th percentile – i.e., 39% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 278,286 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 44 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 59% of its contemporaries.