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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Colorimetric detection of both total genomic and loci-specific DNA methylation from limited DNA inputs
|
|---|---|
| Published in |
Clinical Epigenetics, July 2015
|
| DOI | 10.1186/s13148-015-0100-6 |
| Pubmed ID | |
| Authors |
Eugene J. H. Wee, Thu Ha Ngo, Matt Trau |
| Abstract |
Aberrant DNA methylation marks are potential disease biomarkers, and detecting both total genomic and gene-specific DNA methylation can aid in clinical decisions. While a plethora of methods exist in research, simpler, more convenient alternatives are needed to enhance both routine diagnostics and research. Herein, we describe colorimetric assays using methyl-binding domain (MBD) proteins for rapid and convenient evaluation of total genomic and gene-specific methylation from 50 ng or less DNA input in under 2 h. As little as 5 % methylation differences can be detected and are enhanced by a novel MBD protocol for improved specificity. Our assays could differentiate naïve from de-methylating drug-treated cells and detect the presence of a methylated prostate cancer biomarker in the urine. Finally, the assay was evolved onto disposable screen-printed electrodes for convenient detection of gene-specific methylation in urine. Rapid MBD-based colorimetric and electrochemical approaches to detect DNA methylation from limited samples were successfully demonstrated and applied to clinical samples. We envision that the ease, low sample requirements and speed of these assays could have both clinical and research-wide applications. |
X Demographics
The data shown below were collected from the profiles of 10 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 20% |
| United States | 2 | 20% |
| Australia | 1 | 10% |
| France | 1 | 10% |
| Unknown | 4 | 40% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 7 | 70% |
| Scientists | 3 | 30% |
Mendeley readers
The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 5% |
| Hungary | 1 | 2% |
| United States | 1 | 2% |
| Unknown | 40 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 25% |
| Researcher | 11 | 25% |
| Student > Bachelor | 3 | 7% |
| Student > Doctoral Student | 2 | 5% |
| Professor > Associate Professor | 2 | 5% |
| Other | 7 | 16% |
| Unknown | 8 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 14 | 32% |
| Agricultural and Biological Sciences | 9 | 20% |
| Chemistry | 6 | 14% |
| Engineering | 4 | 9% |
| Medicine and Dentistry | 2 | 5% |
| Other | 0 | 0% |
| Unknown | 9 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 September 2015.
All research outputs
#4,605,955
of 22,816,807 outputs
Outputs from Clinical Epigenetics
#308
of 1,256 outputs
Outputs of similar age
#58,111
of 262,931 outputs
Outputs of similar age from Clinical Epigenetics
#9
of 33 outputs
Altmetric has tracked 22,816,807 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,256 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.5. This one has done well, scoring higher than 75% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 262,931 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 77% of its contemporaries.
We're also able to compare this research output to 33 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.