Title |
Decrease in levels of the evolutionarily conserved microRNA miR-124 affects oligodendrocyte numbers in Zebrafish, Danio rerio
|
---|---|
Published in |
Invertebrate Neuroscience, July 2015
|
DOI | 10.1007/s10158-015-0180-1 |
Pubmed ID | |
Authors |
Jacqueline K. Morris, Anthony Chomyk, Ping Song, Nate Parker, Sadie Deckard, Bruce D. Trapp, Sanjay W. Pimplikar, Ranjan Dutta |
Abstract |
Oligodendrocytes produce multi-lamellar myelin membranes that surround axons in the central nervous system (CNS). Preservation and generation of myelin are potential therapeutic targets for dysmyelinating and demyelinating diseases. MicroRNAs (miRNAs) play a vital role in oligodendrocyte differentiation and overall CNS development. miR-124 is a well-conserved neuronal miRNA with important roles in neuronal differentiation and function. miR-124 levels increase following loss of myelin in both human and rodent brains. While the role of neuronal miR-124 in neurogenesis has been established, its effects on axonal outgrowth and oligodendrocytes are not currently known. We therefore explored the possible effect of selective knockdown of miR-124 in Danio rerio using a morpholino-based knockdown approach. No morphological abnormalities or loss of motor neurons were detected despite loss of axonal outgrowth. Morpholino-based knockdown of miR-124 led to reciprocal increases in mRNA levels of target genes that inhibit axonal and dendritic projections. Importantly, loss of miR-124 led to decreased oligodendrocyte cell numbers and myelination of axonal projections in the ventral hindbrain. Taken together, our results add a new dimension to the existing complexity of neuron-glial relationships and highlight the utility of Danio rerio as a model system to investigate such interactions. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 50% |
Scientists | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 22 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 7 | 32% |
Researcher | 5 | 23% |
Student > Master | 2 | 9% |
Student > Bachelor | 1 | 5% |
Other | 1 | 5% |
Other | 2 | 9% |
Unknown | 4 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 18% |
Neuroscience | 4 | 18% |
Agricultural and Biological Sciences | 4 | 18% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 9% |
Medicine and Dentistry | 2 | 9% |
Other | 2 | 9% |
Unknown | 4 | 18% |