Title |
Advances in animal cell recombinant protein production: GS-NS0 expression system
|
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Published in |
Methods in Cell Science, February 2000
|
DOI | 10.1023/a:1008170710003 |
Pubmed ID | |
Authors |
Louise M. Barnes, Catherine M. Bentley, Alan J. Dickson |
Abstract |
The production of recombinant proteins using mammalian cell expression systems is of growing importance within biotechnology, largely due to the ability of specific mammalian cells to carry out post-translational modifications of the correct fidelity. The Glutamine Synthetase-NS0 system is now one such industrially important expression system.Glutamine synthetase catalyses the formation ofglutamine from glutamate and ammonia. NS0 cellscontain extremely low levels of endogenous glutaminesynthetase activity, therefore exogenous glutaminesynthetase can be used efficiently as a selectablemarker to identify successful transfectants in theabsence of glutamine in the media. In addition, theinclusion of methionine sulphoximine, an inhibitor ofglutamine synthetase activity, enables furtherselection of those clones producing relatively highlevels of transfected glutamine synthetase and henceany heterologous gene which is coupled to it. Theglutamine synthetase system technology has been usedfor research and development purposes during thisdecade and its importance is clearly demonstrated nowthat two therapeutic products produced using thissystem have reached the market place. |
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Geographical breakdown
Country | Count | As % |
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United Kingdom | 3 | 2% |
Switzerland | 2 | 1% |
United States | 2 | 1% |
Australia | 1 | <1% |
Portugal | 1 | <1% |
Canada | 1 | <1% |
Germany | 1 | <1% |
Unknown | 174 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 36 | 19% |
Student > Ph. D. Student | 31 | 17% |
Student > Bachelor | 30 | 16% |
Student > Master | 29 | 16% |
Other | 11 | 6% |
Other | 23 | 12% |
Unknown | 25 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 64 | 35% |
Biochemistry, Genetics and Molecular Biology | 44 | 24% |
Engineering | 15 | 8% |
Chemical Engineering | 10 | 5% |
Immunology and Microbiology | 5 | 3% |
Other | 18 | 10% |
Unknown | 29 | 16% |