| Title |
Targeting breast stem cells with the cancer preventive compounds curcumin and piperine
|
|---|---|
| Published in |
Breast Cancer Research and Treatment, November 2009
|
| DOI | 10.1007/s10549-009-0612-x |
| Pubmed ID | |
| Authors |
Madhuri Kakarala, Dean E. Brenner, Hasan Korkaya, Connie Cheng, Karim Tazi, Christophe Ginestier, Suling Liu, Gabriela Dontu, Max S. Wicha |
| Abstract |
The cancer stem cell hypothesis asserts that malignancies arise in tissue stem and/or progenitor cells through the dysregulation or acquisition of self-renewal. In order to determine whether the dietary polyphenols, curcumin, and piperine are able to modulate the self-renewal of normal and malignant breast stem cells, we examined the effects of these compounds on mammosphere formation, expression of the breast stem cell marker aldehyde dehydrogenase (ALDH), and Wnt signaling. Mammosphere formation assays were performed after curcumin, piperine, and control treatment in unsorted normal breast epithelial cells and normal stem and early progenitor cells, selected by ALDH positivity. Wnt signaling was examined using a Topflash assay. Both curcumin and piperine inhibited mammosphere formation, serial passaging, and percent of ALDH+ cells by 50% at 5 microM and completely at 10 microM concentration in normal and malignant breast cells. There was no effect on cellular differentiation. Wnt signaling was inhibited by both curcumin and piperine by 50% at 5 microM and completely at 10 microM. Curcumin and piperine separately, and in combination, inhibit breast stem cell self-renewal but do not cause toxicity to differentiated cells. These compounds could be potential cancer preventive agents. Mammosphere formation assays may be a quantifiable biomarker to assess cancer preventive agent efficacy and Wnt signaling assessment can be a mechanistic biomarker for use in human clinical trials. |
X Demographics
The data shown below were collected from the profiles of 13 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | 31% |
| United Kingdom | 2 | 15% |
| Canada | 1 | 8% |
| Spain | 1 | 8% |
| Germany | 1 | 8% |
| Unknown | 4 | 31% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 13 | 100% |
| Scientists | 1 | 8% |
Mendeley readers
The data shown below were compiled from readership statistics for 305 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | <1% |
| United States | 1 | <1% |
| Luxembourg | 1 | <1% |
| Unknown | 302 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 53 | 17% |
| Student > Bachelor | 43 | 14% |
| Student > Master | 40 | 13% |
| Researcher | 38 | 12% |
| Other | 15 | 5% |
| Other | 57 | 19% |
| Unknown | 59 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 65 | 21% |
| Biochemistry, Genetics and Molecular Biology | 43 | 14% |
| Medicine and Dentistry | 39 | 13% |
| Chemistry | 24 | 8% |
| Pharmacology, Toxicology and Pharmaceutical Science | 21 | 7% |
| Other | 38 | 12% |
| Unknown | 75 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 57. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 April 2023.
All research outputs
#740,541
of 25,330,051 outputs
Outputs from Breast Cancer Research and Treatment
#72
of 4,980 outputs
Outputs of similar age
#1,806
of 104,157 outputs
Outputs of similar age from Breast Cancer Research and Treatment
#2
of 47 outputs
Altmetric has tracked 25,330,051 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,980 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 7.3. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 104,157 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 98% of its contemporaries.
We're also able to compare this research output to 47 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 97% of its contemporaries.