Title |
Integrated genomic and transcriptomic analysis of human brain metastases identifies alterations of potential clinical significance
|
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Published in |
The Journal of Pathology, August 2015
|
DOI | 10.1002/path.4583 |
Pubmed ID | |
Authors |
Jodi M Saunus, Michael C J Quinn, Ann-Marie Patch, John V Pearson, Peter J Bailey, Katia Nones, Amy E McCart Reed, David Miller, Peter J Wilson, Fares Al-Ejeh, Mythily Mariasegaram, Queenie Lau, Teresa Withers, Rosalind L Jeffree, Lynne E Reid, Leonard Da Silva, Admire Matsika, Colleen M Niland, Margaret C Cummings, Timothy J C Bruxner, Angelika N Christ, Ivon Harliwong, Senel Idrisoglu, Suzanne Manning, Craig Nourse, Ehsan Nourbakhsh, Shivangi Wani, Matthew J Anderson, J Lynn Fink, Oliver Holmes, Stephen Kazakoff, Conrad Leonard, Felicity Newell, Darrin Taylor, Nick Waddell, Scott Wood, Qinying Xu, Karin S Kassahn, Vairavan Narayanan, Nur Aishah Taib, Soo-Hwang Teo, Yock Ping Chow, kConFab, Parmjit S Jat, Sebastian Brandner, Adrienne M Flanagan, Kum Kum Khanna, Georgia Chenevix-Trench, Sean M Grimmond, Peter T Simpson, Nicola Waddell, Sunil R Lakhani |
Abstract |
Treatment options for patients with brain metastases (BM) have limited efficacy and the mortality rate is virtually 100%. Targeted therapy is critically under-utilised, and our understanding of mechanisms underpinning metastatic outgrowth in the brain is limited. To address these deficiencies, we investigated the genomic and transcriptomic landscapes of 36 BM (from breast, lung, melanoma and oesophageal cancers) using DNA copy-number analysis and exome- and RNA-sequencing. The key findings were: (1) identification of novel candidates with possible roles in brain metastasis development, including the significantly mutated genes DSC2, ST7, PIK3R1 and SMC5; and the DNA repair, ERBB/HER signalling, axon guidance and protein kinase-A signalling pathways. (2) Mutational signature analysis was applied to successfully identify the primary cancer type for two BM with unknown origins. (3) Actionable genomic alterations were identified in 31/36 BM (86%). In one case we retrospectively identified ERBB2-amplification representing apparent HER2 status conversion, then confirmed progressive enrichment for HER2-positivity across four consecutive metastatic deposits by IHC and SISH, resulting in the deployment of HER2-targeted therapy for the patient. (4) In the ERBB/HER pathway, ERBB2 expression correlated with ERBB3 (r(2) = 0.496; p<0.0001), and HER3 and HER4 were frequently activated in an independent cohort of 167 archival BM from seven primary cancer types: 57.6% and 52.6% of cases were phospho-HER3(Y1222) or phospho-HER4(Y1162) membrane-positive, respectively. The HER3 ligands NRG1/2 were barely detectable by RNAseq (with NRG1 (8p12) genomic loss in 63.6% breast cancer-BM), suggesting a microenvironmental source of ligand. In summary, this is the first study to characterise the genomic landscapes of BM. The data revealed novel candidates, potential clinical applications for genomic profiling of resectable BM, and highlight the possibility of therapeutically targeting HER3, which is broadly over-expressed and activated in BM independent of primary site and systemic therapy. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 25% |
Australia | 1 | 25% |
Unknown | 2 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 4 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | <1% |
Unknown | 140 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 20 | 14% |
Researcher | 17 | 12% |
Student > Master | 16 | 11% |
Student > Doctoral Student | 12 | 9% |
Student > Bachelor | 12 | 9% |
Other | 32 | 23% |
Unknown | 32 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 38 | 27% |
Biochemistry, Genetics and Molecular Biology | 25 | 18% |
Agricultural and Biological Sciences | 16 | 11% |
Neuroscience | 5 | 4% |
Immunology and Microbiology | 3 | 2% |
Other | 15 | 11% |
Unknown | 39 | 28% |