| Title |
Hepatoprotective effects of Micromeria croatica ethanolic extract against CCl4–induced liver injury in mice
|
|---|---|
| Published in |
BMC Complementary Medicine and Therapies, July 2015
|
| DOI | 10.1186/s12906-015-0763-8 |
| Pubmed ID | |
| Authors |
Sanda Vladimir-Knežević, Olga Cvijanović, Biljana Blažeković, Marija Kindl, Maja Bival Štefan, Robert Domitrović |
| Abstract |
Micromeria croatica (Pers.) Schott is an aromatic plant from Lamiaceae family previously found to possess potent in vitro antioxidant activity which is mainly attributed to the high level of polyphenolic substances. The aim of this study was to investigate the hepatoprotective activity and possible underlying mechanisms of Micromeria croatica ethanolic extract (MC) using a model of carbon tetrachloride (CCl4)-induced liver injury in mice. Male BALB/cN mice were randomly divided into seven groups: control group received saline, MC group received ethanolic extract of M. croatica in 5 % DMSO (100 mg/kg b.w., p.o.), and CCl4 group was administered CCl4 dissolved in corn oil (2 mL/kg, 10 % v/v, ip). MC50, MC200 and MC400 groups were treated with MC orally at doses of 50, 200 and 400 mg/kg once daily for 2 consecutive days, respectively, 6 h after CCl4 intoxication. The reference group received silymarin at dose of 400 mg/kg. At the end of experiment, blood and liver samples were collected for biochemical, histopathological, immunohistochemical and Western blot analyses. In addition, major phenolic compounds in MC were quantified by HPLC-DAD. CCl4 intoxication resulted in liver cells damage and oxidative stress and triggered inflammatory response in mice livers. MC treatment decreased ALT activity and prevented liver necrosis. Improved hepatic antioxidant status was evident by increased Cu/Zn SOD activity and decreased 4-hydroxynonenal (4-HNE) formation in the livers. Concomitantly, nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were overexpressed. The hepatoprotective activity of MC was accompanied by the increase in nuclear factor-kappaB (NF-κB) activation and tumor necrosis factor-alpha (TNF-α) expression, indicating amelioration of hepatic inflammation. Additionally, MC prevented tumor growth factor-β1 (TGF-β1) and α-smooth muscle actin (α-SMA) expression, suggesting the potential for suppression of hepatic fibrogenesis. These results of the present study demonstrated that MC possesses in vivo antioxidant and anti-inflammatory activity and exhibits antifibrotic potential, which are comparable to those of standard hepatoprotective compound silymarin. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Croatia | 1 | 3% |
| United States | 1 | 3% |
| Unknown | 33 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 7 | 20% |
| Student > Ph. D. Student | 5 | 14% |
| Researcher | 3 | 9% |
| Student > Doctoral Student | 2 | 6% |
| Student > Bachelor | 2 | 6% |
| Other | 7 | 20% |
| Unknown | 9 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 20% |
| Pharmacology, Toxicology and Pharmaceutical Science | 6 | 17% |
| Biochemistry, Genetics and Molecular Biology | 3 | 9% |
| Medicine and Dentistry | 3 | 9% |
| Immunology and Microbiology | 1 | 3% |
| Other | 2 | 6% |
| Unknown | 13 | 37% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 July 2015.
All research outputs
#18,418,919
of 22,817,213 outputs
Outputs from BMC Complementary Medicine and Therapies
#2,511
of 3,630 outputs
Outputs of similar age
#189,024
of 262,607 outputs
Outputs of similar age from BMC Complementary Medicine and Therapies
#65
of 97 outputs
Altmetric has tracked 22,817,213 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,630 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.5. This one is in the 17th percentile – i.e., 17% of its peers scored the same or lower than it.
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