| Chapter title |
Analysis of Circulating Tumor DNA
|
|---|---|
| Chapter number | 9 |
| Book title |
Multiple Myeloma
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7865-6_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7864-9, 978-1-4939-7865-6
|
| Authors |
Sridurga Mithraprabhu, Andrew Spencer, Mithraprabhu, Sridurga, Spencer, Andrew |
| Abstract |
Circulating tumor DNA (ctDNA) analysis is currently gaining momentum as an innovative methodology for characterizing the tumor genome and monitoring therapeutic efficacy in the multifocal, genetically and spatially heterogeneous plasma cell malignancy, multiple myeloma (MM). Circulating cell-free DNA (cfDNA), which consists of a combination of DNA derived from both tumor and normal cells, is present in extracellular bodily fluids. The presence of ctDNA within this admixture has been demonstrated recently in MM. In this chapter, we describe the routinely utilized methodology for the extraction and longitudinal analysis of specific mutations present in ctDNA derived from peripheral blood plasma of MM patients. |
X Demographics
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|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 22 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 4 | 18% |
| Student > Master | 4 | 18% |
| Researcher | 4 | 18% |
| Student > Ph. D. Student | 1 | 5% |
| Professor > Associate Professor | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 8 | 36% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 27% |
| Medicine and Dentistry | 6 | 27% |
| Agricultural and Biological Sciences | 2 | 9% |
| Unknown | 8 | 36% |