Title |
The genome of herpesvirus saimiri C488 which is capable of transforming human T cells
|
---|---|
Published in |
Virology, September 2003
|
DOI | 10.1016/s0042-6822(03)00449-5 |
Pubmed ID | |
Authors |
Armin Ensser, Mathias Thurau, Sabine Wittmann, Helmut Fickenscher |
Abstract |
Herpesvirus saimiri (HVS), the rhadinovirus prototype, is apathogenic in the persistently infected natural host, the squirrel monkey, but causes acute T cell leukemia in other New World primate species. In contrast to subgroups A and B, only strains of HVS subgroup C such as C488 are capable of transforming primary human T cells to stable antigen-independent growth in culture. Here, we report the complete 155-kb genome sequence of the transformation-competent HVS strain C488. The A+T-rich unique L-DNA of 113,027 bp encodes at least 77 open reading frames and 5 URNAs. In addition to the viral oncogenes stp and tip, only a few genes including the transactivator orf50 and the glycoprotein orf51 are highly divergent. In a series of new primary HVS isolates, the subgroup-specific divergence of the orf50/orf51 alleles was studied. In these new isolates, the orf50/orf51 alleles of the respective subgroup segregate with the stp and/or tip oncogene alleles, which are essential for transformation. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 3 | 30% |
Professor | 2 | 20% |
Researcher | 2 | 20% |
Student > Bachelor | 2 | 20% |
Unknown | 1 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 30% |
Agricultural and Biological Sciences | 3 | 30% |
Computer Science | 1 | 10% |
Chemistry | 1 | 10% |
Unknown | 2 | 20% |