| Title |
Effect of Roux-en-Y gastric bypass on the distribution and hormone expression of small-intestinal enteroendocrine cells in obese patients with type 2 diabetes
|
|---|---|
| Published in |
Diabetologia, July 2015
|
| DOI | 10.1007/s00125-015-3696-3 |
| Pubmed ID | |
| Authors |
Nicolai A. Rhee, Camilla D. Wahlgren, Jens Pedersen, Brynjulf Mortensen, Ebbe Langholz, Erik P. Wandall, Steffen U. Friis, Peter Vilmann, Sarah J. Paulsen, Viggo B. Kristiansen, Jacob Jelsing, Louise S. Dalbøge, Steen S. Poulsen, Jens J. Holst, Tina Vilsbøll, Filip K. Knop |
| Abstract |
We studied the impact of Roux-en-Y gastric bypass (RYGB) on the density and hormonal gene expression of small-intestinal enteroendocrine cells in obese patients with type 2 diabetes. Twelve patients with diabetes and 11 age- and BMI-matched controls underwent RYGB followed by enteroscopy ~10 months later. Mucosal biopsies taken during surgery and enteroscopy were immunohistochemically stained for glucagon-like peptide-1 (GLP-1), peptide YY (PYY), cholecystokinin (CCK), glucose-dependent insulinotropic polypeptide (GIP) and prohormone convertase 2 (PC2) and the expression of GCG (encoding preproglucagon), PYY, CCK, GIP, GHRL (encoding ghrelin), SCT (encoding secretin), NTS (encoding neurotensin) and NR1H4 (encoding farnesoid X receptor) was evaluated. The density of cells immunoreactive for GLP-1, CCK and GIP increased in patients after RYGB and the density of those immunoreactive for GLP-1, PYY, CCK and PC2 increased in controls. In both groups, GHRL, SCT and GIP mRNA was reduced after RYGB while PYY, CCK, NTS and NR1H4 gene expression was unaltered. GCG mRNA was upregulated in both groups. Numerous alterations in the distribution of enteroendocrine cells and their expression of hormonal genes are seen after RYGB and include increased density of GLP-1-, PYY-, CCK-, GIP- and PC2-positive cells, reduced gene expression of GHRL, SCT and GIP and increased expression of GCG. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 3 | 75% |
| Denmark | 1 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 50% |
| Science communicators (journalists, bloggers, editors) | 1 | 25% |
| Practitioners (doctors, other healthcare professionals) | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 118 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Denmark | 1 | <1% |
| Brazil | 1 | <1% |
| Unknown | 116 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 29 | 25% |
| Researcher | 22 | 19% |
| Student > Master | 11 | 9% |
| Student > Postgraduate | 9 | 8% |
| Student > Bachelor | 7 | 6% |
| Other | 19 | 16% |
| Unknown | 21 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 59 | 50% |
| Agricultural and Biological Sciences | 9 | 8% |
| Biochemistry, Genetics and Molecular Biology | 8 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 3% |
| Engineering | 4 | 3% |
| Other | 9 | 8% |
| Unknown | 25 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 May 2016.
All research outputs
#7,504,088
of 23,576,969 outputs
Outputs from Diabetologia
#2,885
of 5,134 outputs
Outputs of similar age
#86,391
of 264,968 outputs
Outputs of similar age from Diabetologia
#33
of 78 outputs
Altmetric has tracked 23,576,969 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 5,134 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 23.4. This one is in the 43rd percentile – i.e., 43% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 264,968 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.
We're also able to compare this research output to 78 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 57% of its contemporaries.