Title |
Potent Ex Vivo Activity of Naphthoquine and Methylene Blue against Drug-Resistant Clinical Isolates of Plasmodium falciparum and Plasmodium vivax
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Published in |
Antimicrobial Agents and Chemotherapy, July 2015
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DOI | 10.1128/aac.00874-15 |
Pubmed ID | |
Authors |
Grennady Wirjanata, Boni F. Sebayang, Ferryanto Chalfein, Prayoga, Irene Handayuni, Leily Trianty, Enny Kenangalem, Rintis Noviyanti, Brice Campo, Jeanne Rini Poespoprodjo, Jörg J. Möhrle, Ric N. Price, Jutta Marfurt |
Abstract |
The 4-aminoquinoline naphthoquine (NQ) and the thiazine dye methylene blue (MB) have potent in vitro efficacy against P. falciparum, but susceptibility data for P. vivax are limited. The species- and stage-specific ex vivo activities of NQ and MB were assessed using a modified schizont maturation assay on clinical field isolates from Papua, Indonesia, where multidrug resistant P. falciparum and P. vivax are prevalent. Both compounds were highly active against P. falciparum (median IC50 of NQ = 8.0 nM [Range: 2.6-71.8 nM] and MB = 1.6 nM [0.2-7.0 nM]) and P. vivax (NQ = 7.8 nM [1.5-34.2] and MB =1.2 nM [0.4-4.3]). Stage-specific drug susceptibility assays revealed significantly greater IC50s in parasites exposed at trophozoite compared to ring stage for NQ in P. falciparum (26.5 versus 5.1 nM, p=0.021) and P. vivax (341.6 versus 6.5 nM, p=0.021), and for MB in P. vivax (10.1 versus 1.6 nM, p=0.010). The excellent ex vivo activity of NQ and MB against both P. falciparum and P. vivax highlights their potential utility for the treatment of multidrug resistant malaria in areas endemic for both species. |
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Mendeley readers
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Student > Bachelor | 2 | 7% |
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Environmental Science | 2 | 7% |
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