Title |
Genome sequence of Clostridium sporogenes DSM 795T, an amino acid-degrading, nontoxic surrogate of neurotoxin-producing Clostridium botulinum
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Published in |
Environmental Microbiome, July 2015
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DOI | 10.1186/s40793-015-0016-y |
Pubmed ID | |
Authors |
Anja Poehlein, Karin Riegel, Sandra M König, Andreas Leimbach, Rolf Daniel, Peter Dürre |
Abstract |
Clostridium sporogenes DSM 795 is the type strain of the species Clostridium sporogenes, first described by Metchnikoff in 1908. It is a Gram-positive, rod-shaped, anaerobic bacterium isolated from human faeces and belongs to the proteolytic branch of clostridia. C. sporogenes attracts special interest because of its potential use in a bacterial therapy for certain cancer types. Genome sequencing and annotation revealed several gene clusters coding for proteins involved in anaerobic degradation of amino acids, such as glycine and betaine via Stickland reaction. Genome comparison showed that C. sporogenes is closely related to C. botulinum. The genome of C. sporogenes DSM 795 consists of a circular chromosome of 4.1 Mb with an overall GC content of 27.81 mol% harboring 3,744 protein-coding genes, and 80 RNAs. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 1 | 2% |
Unknown | 55 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 13 | 23% |
Student > Ph. D. Student | 11 | 20% |
Researcher | 6 | 11% |
Student > Master | 6 | 11% |
Student > Postgraduate | 3 | 5% |
Other | 6 | 11% |
Unknown | 11 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 13 | 23% |
Agricultural and Biological Sciences | 9 | 16% |
Immunology and Microbiology | 6 | 11% |
Engineering | 4 | 7% |
Chemistry | 3 | 5% |
Other | 8 | 14% |
Unknown | 13 | 23% |