| Title |
Construction of a reverse genetic system for porcine astrovirus
|
|---|---|
| Published in |
Archives of Virology, February 2018
|
| DOI | 10.1007/s00705-018-3771-4 |
| Pubmed ID | |
| Authors |
Yifeng Qin, Qingli Fang, Huan Liu, Chengyuan Ji, Ying Chen, Kang Ouyang, Zuzhang Wei, Weijian Huang |
| Abstract |
In order to construct a full-length infectious cDNA clone of porcine astrovirus, three fragments covering the complete genome of PAstV1-GX1 strain were amplified by RT-PCR. All three PCR-amplified fragments were cloned into T-Vector pMD19 (Simple), and subsequently assembled into a full-length cDNA clone by subcloning. A silent nucleotide change creating a PstI site was engineered into the full-length cDNA clone to distinguish the rescued virus from the parental virus. Upon transfection of BHK-21 cells with the in vitro transcripts of both the original and constructed cDNAs, typical cytopathic effects were observed on PK-15 cells after serial passaging of the cell supernatant. The construction and recovery of the infectious cDNA clone of porcine astrovirus will provide a valuable experimental system to study the genome function and pathogenesis of astroviruses. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 14 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 2 | 14% |
| Student > Ph. D. Student | 2 | 14% |
| Unspecified | 1 | 7% |
| Researcher | 1 | 7% |
| Other | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 7 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 21% |
| Unspecified | 1 | 7% |
| Veterinary Science and Veterinary Medicine | 1 | 7% |
| Agricultural and Biological Sciences | 1 | 7% |
| Immunology and Microbiology | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 7 | 50% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 May 2018.
All research outputs
#20,516,195
of 23,083,773 outputs
Outputs from Archives of Virology
#3,420
of 4,215 outputs
Outputs of similar age
#408,354
of 474,615 outputs
Outputs of similar age from Archives of Virology
#74
of 96 outputs
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We're also able to compare this research output to 96 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.