| Title |
Establishment of a multiplex real-time RT-PCR assay for rapid identification of H6 subtype avian influenza viruses
|
|---|---|
| Published in |
Archives of Virology, February 2018
|
| DOI | 10.1007/s00705-018-3773-2 |
| Pubmed ID | |
| Authors |
Fan Yang, Haibo Wu, Fumin Liu, Xiangyun Lu, Xiuming Peng, Nanping Wu |
| Abstract |
The H6 subtype avian influenza viruses (AIVs) possess the capacity for zoonotic transmission from avian species to humans. Establishment of a specific, rapid and sensitive method to screen H6 AIVs is necessary. Based on the conserved domain of the matrix and H6 AIV hemagglutinin genes, two TaqMan minor-groove-binder probes and multiplex real-time RT-PCR primers were designed in this study. The multiplex real-time RT-PCR assay developed in this study had high specificity and repeatability and a detection limit of 30 copies per reaction. This rapid diagnostic method will be useful for clinical detection and surveillance of H6 AIVs in China. |
Mendeley readers
The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 16 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 19% |
| Student > Master | 3 | 19% |
| Lecturer | 1 | 6% |
| Professor | 1 | 6% |
| Unspecified | 1 | 6% |
| Other | 4 | 25% |
| Unknown | 3 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 25% |
| Biochemistry, Genetics and Molecular Biology | 2 | 13% |
| Immunology and Microbiology | 2 | 13% |
| Unspecified | 1 | 6% |
| Chemical Engineering | 1 | 6% |
| Other | 3 | 19% |
| Unknown | 3 | 19% |