| Title |
Improved properties of bone and cartilage tissue from 3D inkjet-bioprinted human mesenchymal stem cells by simultaneous deposition and photocrosslinking in PEG-GelMA
|
|---|---|
| Published in |
Biotechnology Techniques, July 2015
|
| DOI | 10.1007/s10529-015-1921-2 |
| Pubmed ID | |
| Authors |
Guifang Gao, Arndt F. Schilling, Karen Hubbell, Tomo Yonezawa, Danh Truong, Yi Hong, Guohao Dai, Xiaofeng Cui |
| Abstract |
Bioprinting of bone and cartilage suffers from low mechanical properties. Here we have developed a unique inkjet bioprinting approach of creating mechanically strong bone and cartilage tissue constructs using poly(ethylene glycol) dimethacrylate, gelatin methacrylate, and human MSCs. The printed hMSCs were evenly distributed in the polymerized PEG-GelMA scaffold during layer-by-layer assembly. The procedure showed a good biocompatibility with >80% of the cells surviving the printing process and the resulting constructs provided strong mechanical support to the embedded cells. The printed mesenchymal stem cells showed an excellent osteogenic and chondrogenic differentiation capacity. Both osteogenic and chondrogenic differentiation as determined by specific gene and protein expression analysis (RUNX2, SP7, DLX5, ALPL, Col1A1, IBSP, BGLAP, SPP1, Col10A1, MMP13, SOX9, Col2A1, ACAN) was improved by PEG-GelMA in comparison to PEG alone. These observations were consistent with the histological evaluation. Inkjet bioprinted-hMSCs in simultaneously photocrosslinked PEG-GelMA hydrogel scaffolds demonstrated an improvement of mechanical properties and osteogenic and chondrogenic differentiation, suggesting its promising potential for usage in bone and cartilage tissue engineering. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 439 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 2 | <1% |
| United Kingdom | 1 | <1% |
| United States | 1 | <1% |
| Canada | 1 | <1% |
| Unknown | 434 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 92 | 21% |
| Student > Master | 78 | 18% |
| Student > Bachelor | 38 | 9% |
| Researcher | 37 | 8% |
| Student > Doctoral Student | 30 | 7% |
| Other | 52 | 12% |
| Unknown | 112 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Engineering | 92 | 21% |
| Materials Science | 43 | 10% |
| Agricultural and Biological Sciences | 41 | 9% |
| Biochemistry, Genetics and Molecular Biology | 35 | 8% |
| Medicine and Dentistry | 28 | 6% |
| Other | 65 | 15% |
| Unknown | 135 | 31% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 February 2023.
All research outputs
#8,270,860
of 25,394,764 outputs
Outputs from Biotechnology Techniques
#801
of 2,763 outputs
Outputs of similar age
#90,361
of 275,368 outputs
Outputs of similar age from Biotechnology Techniques
#5
of 37 outputs
Altmetric has tracked 25,394,764 research outputs across all sources so far. This one has received more attention than most of these and is in the 66th percentile.
So far Altmetric has tracked 2,763 research outputs from this source. They receive a mean Attention Score of 3.9. This one has gotten more attention than average, scoring higher than 70% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 275,368 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.
We're also able to compare this research output to 37 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 78% of its contemporaries.