| Title |
Molecular diagnosis of natural fasciolosis by DNA detection in sheep faeces
|
|---|---|
| Published in |
Acta Parasitologica, March 2015
|
| DOI | 10.1515/ap-2015-0030 |
| Pubmed ID | |
| Authors |
Silvana Carnevale, María Laura Pantano, Laura Kamenetzky, Jorge Bruno Malandrini, Claudia Cecilia Soria, Jorge Néstor Velásquez |
| Abstract |
Fasciolosis is an important parasitic zoonosis considered the most important helminth infection of ruminants in tropical countries. The aim of this study was to develop a PCR assay for the sensitive and specific detection of F. hepatica in formalin preserved sheep faeces. A 405-bp fragment of the cytochrome c oxidase subunit 1 gene of F. hepatica was amplified from stool samples of infected sheep. The PCR assay showed a detection limit of 20 pg of F. hepatica DNA. No cross-reactions were observed with samples containing coccidian oocysts or gastrointestinal nematodes eggs. Our PCR technique showed to be effective for specific detection of F. hepatica infections in sheep. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Uruguay | 1 | 4% |
| Unknown | 23 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 4 | 17% |
| Student > Doctoral Student | 4 | 17% |
| Student > Bachelor | 4 | 17% |
| Professor | 2 | 8% |
| Student > Ph. D. Student | 2 | 8% |
| Other | 4 | 17% |
| Unknown | 4 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 29% |
| Veterinary Science and Veterinary Medicine | 6 | 25% |
| Medicine and Dentistry | 3 | 13% |
| Biochemistry, Genetics and Molecular Biology | 1 | 4% |
| Unknown | 7 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 July 2015.
All research outputs
#22,756,649
of 25,371,288 outputs
Outputs from Acta Parasitologica
#481
of 735 outputs
Outputs of similar age
#239,263
of 277,727 outputs
Outputs of similar age from Acta Parasitologica
#6
of 15 outputs
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