| Title |
Stage-dependent piRNAs in chicken implicated roles in modulating male germ cell development
|
|---|---|
| Published in |
BMC Genomics, June 2018
|
| DOI | 10.1186/s12864-018-4820-9 |
| Pubmed ID | |
| Authors |
Kai-Wei Chang, Yen-Tzu Tseng, Yi-Chen Chen, Chih-Yun Yu, Hung-Fu Liao, Yi-Chun Chen, Yu-Fan Evan Tu, Shinn-Chih Wu, I-Hsuan Liu, Marina Pinskaya, Antonin Morillon, Bertrand Pain, Shau-Ping Lin |
| Abstract |
The PIWI/piRNA pathway is a conserved machinery important for germ cell development and fertility. This piRNA-guided molecular machinery is best known for repressing derepressed transposable elements (TE) during epigenomic reprogramming. The extent to which piRNAs are involved in modulating transcripts beyond TEs still need to be clarified, and it may be a stage-dependent event. We chose chicken germline as a study model because of the significantly lower TE complexity in the chicken genome compared to mammalian species. We generated high-confidence piRNA candidates in various stages across chicken germline development by 3'-end-methylation-enriched small RNA sequencing and in-house bioinformatics analysis. We observed a significant developmental stage-dependent loss of TE association and a shifting of the ping-pong cycle signatures. Moreover, the stage-dependent reciprocal abundance of LINE retrotransposons, CR1-C, and its associated piRNAs implicated the developmental stage-dependent role of piRNA machinery. The stage dependency of piRNA expression and its potential functions can be better addressed by analyzing the piRNA precursors/clusters. Interestingly, the new piRNA clusters identified from embryonic chicken testes revealed evolutionary conservation between chickens and mammals, which was previously thought to not exist. In this report, we provided an original chicken RNA resource and proposed an analytical methodology that can be used to investigate stage-dependent changes in piRNA compositions and their potential roles in TE regulation and beyond, and also revealed possible conserved functions of piRNAs in developing germ cells. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 2 | 25% |
| United States | 1 | 13% |
| Unknown | 5 | 63% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 50% |
| Scientists | 3 | 38% |
| Science communicators (journalists, bloggers, editors) | 1 | 13% |
Mendeley readers
The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 16 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 25% |
| Other | 2 | 13% |
| Student > Master | 2 | 13% |
| Lecturer | 1 | 6% |
| Student > Ph. D. Student | 1 | 6% |
| Other | 2 | 13% |
| Unknown | 4 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 38% |
| Agricultural and Biological Sciences | 5 | 31% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
| Unknown | 4 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 December 2018.
All research outputs
#7,253,504
of 23,085,832 outputs
Outputs from BMC Genomics
#3,416
of 10,705 outputs
Outputs of similar age
#125,424
of 330,319 outputs
Outputs of similar age from BMC Genomics
#85
of 257 outputs
Altmetric has tracked 23,085,832 research outputs across all sources so far. This one has received more attention than most of these and is in the 68th percentile.
So far Altmetric has tracked 10,705 research outputs from this source. They receive a mean Attention Score of 4.7. This one has gotten more attention than average, scoring higher than 67% of its peers.
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We're also able to compare this research output to 257 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.