Title |
“Artificial micro organs”—a microfluidic device for dielectrophoretic assembly of liver sinusoids
|
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Published in |
Biomedical Microdevices, February 2011
|
DOI | 10.1007/s10544-011-9517-7 |
Pubmed ID | |
Authors |
Julia Schütte, Britta Hagmeyer, Felix Holzner, Massimo Kubon, Simon Werner, Christian Freudigmann, Karin Benz, Jan Böttger, Rolf Gebhardt, Holger Becker, Martin Stelzle |
Abstract |
In order to study possible toxic side effects of potential drug compounds in vitro a reliable test system is needed. Predicting liver toxicity presents a major challenge of particular importance as liver cells grown in a cell culture suffer from a rapid loss of their liver specific functions. Therefore we are developing a new microfluidic test system for liver toxicity. This test system is based on an organ-like liver 3D co-culture of hepatocytes and endothelial cells. We devised a microfluidic chip featuring cell culture chambers with integrated electrodes for the assembly of liver sinusoids by dielectrophoresis. Fluid channels enable an organ-like perfusion with culture media and test compounds. Different chamber designs were studied and optimized with regard to dielectrophoretic force distribution, hydrodynamic flow profile, and cell trapping rate using numeric simulations. Based on simulation results a microchip was injection-moulded from COP. This chip allowed the assembly of viable hepatocytes and endothelial cells in a sinusoid-like fashion. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 1 | <1% |
Switzerland | 1 | <1% |
Iran, Islamic Republic of | 1 | <1% |
Denmark | 1 | <1% |
Spain | 1 | <1% |
United States | 1 | <1% |
Unknown | 118 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 39 | 31% |
Researcher | 22 | 18% |
Student > Master | 18 | 15% |
Student > Doctoral Student | 11 | 9% |
Student > Bachelor | 6 | 5% |
Other | 11 | 9% |
Unknown | 17 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Engineering | 31 | 25% |
Agricultural and Biological Sciences | 29 | 23% |
Biochemistry, Genetics and Molecular Biology | 14 | 11% |
Pharmacology, Toxicology and Pharmaceutical Science | 6 | 5% |
Physics and Astronomy | 5 | 4% |
Other | 17 | 14% |
Unknown | 22 | 18% |