| Title |
Fluorouracil induces autophagy-related gastric carcinoma cell death through Beclin-1 upregulation by miR-30 suppression
|
|---|---|
| Published in |
Tumor Biology, July 2015
|
| DOI | 10.1007/s13277-015-3775-6 |
| Pubmed ID | |
| Authors |
Chun Yang, Yong Pan |
| Abstract |
The molecular mechanisms underlying the anti-cancer effects of chemotherapy drugs are not completely understood. Here, we studied the effects of fluorouracil (5-FU) on gastric carcinoma (GC) cells. We found that 5-FU dose-dependently inhibited the growth of GC cells, in either a cell counting kit-8 (CCK-8) assay or a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Moreover, autophagy-associated protein 6 (ATG6) or Beclin-1 was dose-dependently activated by 5-FU in GC cells. Further, microRNA (miR)-30 was found to be regulated by 5-FU, and bioinformatics analysis showed that miR-30 targeted the 3'-UTR of Beclin-1 to inhibit its translation. Together, these data suggest that 5-FU may suppress miR-30 to upregulate Beclin-1 to induce autophagic cell death and cell proliferation arrest in GC cells. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 2 | 18% |
| Researcher | 2 | 18% |
| Professor | 1 | 9% |
| Student > Doctoral Student | 1 | 9% |
| Student > Master | 1 | 9% |
| Other | 1 | 9% |
| Unknown | 3 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 27% |
| Medicine and Dentistry | 2 | 18% |
| Agricultural and Biological Sciences | 2 | 18% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 9% |
| Unknown | 3 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 July 2015.
All research outputs
#18,420,033
of 22,818,766 outputs
Outputs from Tumor Biology
#1,369
of 2,622 outputs
Outputs of similar age
#189,156
of 263,272 outputs
Outputs of similar age from Tumor Biology
#73
of 170 outputs
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