Title |
Comprehensive transcriptome and improved genome annotation of Bacillus licheniformis WX‐02
|
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Published in |
Febs Letters, July 2015
|
DOI | 10.1016/j.febslet.2015.07.029 |
Pubmed ID | |
Authors |
Jing Guo, Gang Cheng, Xiang-Yong Gou, Feng Xing, Sen Li, Yi-Chao Han, Long Wang, Jia-Ming Song, Cheng-Cheng Shu, Shou-Wen Chen, Ling-Ling Chen |
Abstract |
The updated genome of Bacillus licheniformis WX-02 comprises a circular chromosome of 4,286,821 base-pairs containing 4,512 protein-coding genes. We applied strand-specific RNA-sequencing to explore the transcriptome profiles of Bacillus licheniformis WX-02 under normal and high-salt conditions (NaCl 6%). We identified 2,381 co-expressed gene pairs constituting 871 operon structures. In addition, 1,169 antisense transcripts and 90 small RNAs were detected. Systematic comparison of differentially expressed genes under different conditions revealed that genes involved in multiple functions were significantly repressed in long-term high salt adaptation process. Genes related to promotion of glutamic acid synthesis were activated by 6% NaCl, potentially explaining the high yield of γ-PGA under salt condition. This study will be useful for the optimization of crucial metabolic activities in this bacterium. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 25 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 7 | 28% |
Student > Master | 4 | 16% |
Student > Bachelor | 2 | 8% |
Student > Postgraduate | 2 | 8% |
Student > Doctoral Student | 1 | 4% |
Other | 4 | 16% |
Unknown | 5 | 20% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 8 | 32% |
Biochemistry, Genetics and Molecular Biology | 4 | 16% |
Engineering | 2 | 8% |
Environmental Science | 1 | 4% |
Immunology and Microbiology | 1 | 4% |
Other | 1 | 4% |
Unknown | 8 | 32% |