| Title |
The soybean R2R3 MYB transcription factor GmMYB100 negatively regulates plant flavonoid biosynthesis
|
|---|---|
| Published in |
Plant Molecular Biology, August 2015
|
| DOI | 10.1007/s11103-015-0349-3 |
| Pubmed ID | |
| Authors |
Junhui Yan, Biao Wang, Yunpeng Zhong, Luming Yao, Linjing Cheng, Tianlong Wu |
| Abstract |
Soybean flavonoids, a group of important signaling molecules in plant-environment interaction, ubiquitously exist in soybean and are tightly regulated by many genes. Here we reported that GmMYB100, a gene encoding a R2R3 MYB transcription factor, is involved in soybean flavonoid biosynthesis. GmMYB100 is mainly expressed in flowers, leaves and immature embryo, and its level is decreased after pod ripening. Subcellular localization assay indicates that GmMYB100 is a nuclear protein. GmMYB100 has transactivation ability revealed by a yeast functional assay; whereas bioinformatic analysis suggests that GmMYB100 has a negative function in flavonoid biosynthesis. GmMYB100-overexpression represses the transcript levels of flavonoid-related genes in transgenic soybean hairy roots and Arabidopsis, and inhibits isoflavonoid (soybean) and flavonol (Arabidopsis) production in transgenic plants. Furthermore, the transcript levels of six flavonoid-related genes and flavonoid (isoflavonoid and flavone aglycones) accumulation are elevated in the GmMYB100-RNAi transgenic hairy roots. We also demonstrate that GmMYB100 protein depresses the promoter activities of soybean chalcone synthase and chalcone isomerase. These findings indicate that GmMYB100 is a negative regulator in soybean flavonoid biosynthesis pathway. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 48 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 2% |
| Brazil | 1 | 2% |
| Unknown | 46 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 19% |
| Researcher | 8 | 17% |
| Student > Master | 8 | 17% |
| Student > Doctoral Student | 3 | 6% |
| Student > Bachelor | 2 | 4% |
| Other | 6 | 13% |
| Unknown | 12 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 16 | 33% |
| Biochemistry, Genetics and Molecular Biology | 13 | 27% |
| Unspecified | 1 | 2% |
| Engineering | 1 | 2% |
| Unknown | 17 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 August 2015.
All research outputs
#18,420,033
of 22,818,766 outputs
Outputs from Plant Molecular Biology
#2,492
of 2,846 outputs
Outputs of similar age
#189,940
of 264,249 outputs
Outputs of similar age from Plant Molecular Biology
#20
of 27 outputs
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