Title |
Detection of Anaplasma phagocytophilum in Ixodes ricinus ticks from Norway using a realtime PCR assay targeting the Anaplasma citrate synthase gene gltA
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Published in |
BMC Microbiology, August 2015
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DOI | 10.1186/s12866-015-0486-5 |
Pubmed ID | |
Authors |
Anna J. Henningsson, Dag Hvidsten, Bjørn-Erik Kristiansen, Andreas Matussek, Snorre Stuen, Andrew Jenkins |
Abstract |
A TaqMan real-time PCR assay targeting the Anaplasma citrate synthase gene, gltA, was developed and used for detection of Anaplasma phagocytophilum in 765 Ixodes ricinus ticks collected from dogs and cats in northern Norway (n = 669) and Telemark county in southern Norway (n = 96). Among the ticks from northern Norway the prevalence of A. phagocytophilum was 3.0 %, while the prevalence in southern Norway was 2.1 % (p = 0.63). The gltA PCR assay showed a high analytical sensitivity (30 genomic units) and efficiency (98.5 %), and its utility in clinical diagnostics should be evaluated in future studies. This is the first report of A. phagocytophilum occurrence in ticks collected north of the Arctic Circle in Norway. The prevalence is comparable to that found in Telemark county in southern Norway. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Norway | 1 | 17% |
Germany | 1 | 17% |
United Kingdom | 1 | 17% |
Unknown | 3 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 6 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 3% |
Unknown | 33 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 7 | 21% |
Researcher | 6 | 18% |
Student > Bachelor | 3 | 9% |
Other | 2 | 6% |
Student > Master | 2 | 6% |
Other | 3 | 9% |
Unknown | 11 | 32% |
Readers by discipline | Count | As % |
---|---|---|
Veterinary Science and Veterinary Medicine | 9 | 26% |
Agricultural and Biological Sciences | 8 | 24% |
Medicine and Dentistry | 2 | 6% |
Biochemistry, Genetics and Molecular Biology | 1 | 3% |
Social Sciences | 1 | 3% |
Other | 1 | 3% |
Unknown | 12 | 35% |