| Title |
Gene expression changes by high-polyphenols cocoa powder intake: a randomized crossover clinical study
|
|---|---|
| Published in |
European Journal of Nutrition, June 2018
|
| DOI | 10.1007/s00394-018-1736-8 |
| Pubmed ID | |
| Authors |
P. K. Barrera-Reyes, N. Hernández-Ramírez, J. Cortés, L. Poquet, K. Redeuil, C. Rangel-Escareño, M. Kussmann, I. Silva-Zolezzi, M. E. Tejero |
| Abstract |
To assess the effect of the intake of a single dose of high-polyphenols cocoa on gene expression in peripheral mononuclear cells (PBMCs), and analyze conjugated (-)-epicatechin metabolites in plasma, which may be related with an antioxidant response in healthy human. A randomized, controlled, double-blind, cross-over, clinical trial in healthy young adults who consumed a single dose of high-polyphenols cocoa powder and maltodextrins as control, with a one-week washout period. Analysis of circulating metabolites, plasma antioxidant capacity and gene expression changes in PBMCs were performed under fasting conditions and 2-h after treatment using microarray in a subsample. Pathway analysis was conducted using Ingenuity Pathway Analysis (IPA). Twenty healthy participants (9 F) were included in the study. A significant increase in circulating (-)-epicatechin metabolites was found after cocoa intake in all participants without related changes in antioxidant capacity of plasma. The metabolites profile slightly varied across subjects. Treatments triggered different transcriptional changes in PBMC. A group of 98 genes showed changes in expression after cocoa treatment, while only 18 were modified by control. Differentially expressed genes included inflammatory cytokines and other molecules involved in redox balance. Gene and network analysis after cocoa intake converged in functions annotated as decreased production of reactive oxygen species (p = 9.58E-04), decreased leukocyte activation (p = 4E-03) and calcium mobilization (p = 2.51E-05). No association was found between conjugated metabolites in plasma and antioxidant capacity. Changes in PBMCs gene expression suggest anti-inflammatory effects. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Practitioners (doctors, other healthcare professionals) | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 90 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 90 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 14 | 16% |
| Student > Master | 13 | 14% |
| Other | 5 | 6% |
| Researcher | 5 | 6% |
| Student > Postgraduate | 4 | 4% |
| Other | 11 | 12% |
| Unknown | 38 | 42% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 12 | 13% |
| Medicine and Dentistry | 10 | 11% |
| Agricultural and Biological Sciences | 6 | 7% |
| Nursing and Health Professions | 5 | 6% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 3% |
| Other | 11 | 12% |
| Unknown | 43 | 48% |
Attention Score in Context
This research output has an Altmetric Attention Score of 29. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 May 2022.
All research outputs
#1,174,898
of 23,090,520 outputs
Outputs from European Journal of Nutrition
#306
of 2,411 outputs
Outputs of similar age
#27,647
of 328,957 outputs
Outputs of similar age from European Journal of Nutrition
#10
of 74 outputs
Altmetric has tracked 23,090,520 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 94th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,411 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 21.4. This one has done well, scoring higher than 87% of its peers.
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We're also able to compare this research output to 74 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 86% of its contemporaries.