Title |
Mushroom extract inhibits ultraviolet B-induced cellular senescence in human keratinocytes
|
---|---|
Published in |
Methods in Cell Science, June 2018
|
DOI | 10.1007/s10616-018-0229-1 |
Pubmed ID | |
Authors |
Zhao Chong, Haruka Matsuo, Mai Kuroda, Shuntaro Yamashita, Gopal Prasad Parajuli, Hira Kaji Manandhar, Kuniyoshi Shimizu, Yoshinori Katakura |
Abstract |
Mushrooms possess various bioactivities and are used as nutritional supplements and medicinal products. Twenty-nine bioactive components have been extracted recently from mushrooms grown in Nepal. In this study, we evaluated the ability of these mushroom extracts to augment SIRT1, a mammalian SIR2 homologue localized in cytosol and nuclei. We established a system for screening food ingredients that augment the SIRT1 promoter in HaCaT cells, and identified a SIRT1-augmenting mushroom extract (number 28, Trametes versicolor). UVB irradiation induced cellular senescence in HaCaT cells, as evidenced by increased activity and expression of cellular senescence markers including senescence-associated β-galactosidase, p21, p16, phosphorylated p38, and γH2AX. Results clearly showed that the mushroom extract (No. 28) suppressed the ultraviolet B irradiation-induced cellular senescence in HaCaT cells possibly through augmenting SIRT1 expression. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 16 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 3 | 19% |
Professor | 2 | 13% |
Student > Doctoral Student | 1 | 6% |
Student > Ph. D. Student | 1 | 6% |
Student > Master | 1 | 6% |
Other | 1 | 6% |
Unknown | 7 | 44% |
Readers by discipline | Count | As % |
---|---|---|
Social Sciences | 2 | 13% |
Environmental Science | 1 | 6% |
Business, Management and Accounting | 1 | 6% |
Biochemistry, Genetics and Molecular Biology | 1 | 6% |
Agricultural and Biological Sciences | 1 | 6% |
Other | 1 | 6% |
Unknown | 9 | 56% |