| Title |
Transgene expression in tick cells using Agrobacterium tumefaciens
|
|---|---|
| Published in |
Experimental and Applied Acarology, July 2015
|
| DOI | 10.1007/s10493-015-9949-5 |
| Pubmed ID | |
| Authors |
Erik Machado-Ferreira, Emilia Balsemão-Pires, Gabrielle Dietrich, Andrias Hojgaard, Vinicius F. Vizzoni, Glen Scoles, Lesley Bell-Sakyi, Joseph Piesman, Nordin S. Zeidner, Carlos A. G. Soares |
| Abstract |
Ticks transmit infectious agents to humans and other animals. Genetic manipulation of vectors like ticks could enhance the development of alternative disease control strategies. Transgene expression using the phytopathogen Agrobacterium tumefaciens has been shown to promote the genetic modification of non-plant cells. In the present work we developed T-DNA constructs for A. tumefaciens to mediate transgene expression in HeLa cells as well as Rhipicephalus microplus tick cells. Translational fusions eGfp:eGfp or Salp15:eGfp, including the enhanced-green fluorescent protein and the Ixodes scapularis salivary factor SALP15 genes, were constructed using the CaMV 35S (cauliflower mosaic virus) promoter, "PBm" tick promoter (R. microplus pyrethroid metabolizing esterase gene) or the Simian Virus SV40 promoter. Confocal microscopy, RT-PCR and Western-blot assays demonstrated transgene(s) expression in both cell lines. Transgene expression was also achieved in vivo, in both R. microplus and I. scapularis larvae utilizing a soaking method including the A. tumefaciens donor cells and confirmed by nested-RT-PCR showing eGfp or Salp15 poly-A-mRNA(s). This strategy opens up a new avenue to express exogenous genes in ticks and represents a potential breakthrough for the study of tick-host pathophysiology. |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Algeria | 1 | 4% |
| Unknown | 27 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 9 | 32% |
| Student > Master | 4 | 14% |
| Student > Bachelor | 3 | 11% |
| Student > Ph. D. Student | 3 | 11% |
| Professor > Associate Professor | 2 | 7% |
| Other | 2 | 7% |
| Unknown | 5 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 9 | 32% |
| Agricultural and Biological Sciences | 7 | 25% |
| Veterinary Science and Veterinary Medicine | 2 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
| Immunology and Microbiology | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 8 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 July 2015.
All research outputs
#16,069,695
of 23,849,058 outputs
Outputs from Experimental and Applied Acarology
#502
of 914 outputs
Outputs of similar age
#157,035
of 265,973 outputs
Outputs of similar age from Experimental and Applied Acarology
#7
of 23 outputs
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So far Altmetric has tracked 914 research outputs from this source. They receive a mean Attention Score of 3.8. This one is in the 35th percentile – i.e., 35% of its peers scored the same or lower than it.
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We're also able to compare this research output to 23 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 56% of its contemporaries.