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Rapid, culture-independent, optical diagnostics of centrifugally captured bacteria from urine samples

Overview of attention for article published in Biomicrofluidics, August 2015
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#23 of 833)
  • High Attention Score compared to outputs of the same age (96th percentile)
  • High Attention Score compared to outputs of the same age and source (95th percentile)

Mentioned by

news
6 news outlets
blogs
2 blogs
twitter
3 X users
patent
1 patent
facebook
1 Facebook page

Citations

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35 Dimensions

Readers on

mendeley
71 Mendeley
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Title
Rapid, culture-independent, optical diagnostics of centrifugally captured bacteria from urine samples
Published in
Biomicrofluidics, August 2015
DOI 10.1063/1.4928070
Pubmed ID
Authors

Ulrich-Christian Schröder, Frank Bokeloh, Mary O'Sullivan, Uwe Glaser, Katharina Wolf, Wolfgang Pfister, Jürgen Popp, Jens Ducrée, Ute Neugebauer

Abstract

This work presents a polymeric centrifugal microfluidic platform for the rapid and sensitive identification of bacteria directly from urine, thus eliminating time-consuming cultivation steps. This "Lab-on-a-Disc" platform utilizes the rotationally induced centrifugal field to efficiently capture bacteria directly from suspension within a glass-polymer hybrid chip. Once trapped in an array of small V-shaped structures, the bacteria are readily available for spectroscopic characterization, such as Raman spectroscopic fingerprinting, providing valuable information on the characteristics of the captured bacteria. Utilising fluorescence microscopy, quantification of the bacterial load has been achieved for concentrations above 2 × 10(-7) cells ml(-1) within a 4 μl sample. As a pilot application, we characterize urine samples from patients with urinary tract infections. Following minimal sample preparation, Raman spectra of the bacteria are recorded following centrifugal capture in stopped-flow sedimentation mode. Utilizing advanced analysis algorithms, including extended multiplicative scattering correction, high-quality Raman spectra of different pathogens, such as Escherichia coli or Enterococcus faecalis, are obtained from the analyzed patient samples. The whole procedure, including sample preparation, requires about 1 h to obtain a valuable result, marking a significant reduction in diagnosis time when compared to the 24 h and more typically required for standard microbiological methods. As this cost-efficient centrifugal cartridge can be operated using low-complexity, widely automated instrumentation, while providing valuable bacterial identification in urine samples in a greatly reduced time-period, our opto-microfluidic Lab-on-a-Disc device demonstrates great potential for next-generation patient diagnostics at the of point-of-care.

X Demographics

X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 71 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Canada 1 1%
Switzerland 1 1%
Unknown 69 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 16 23%
Researcher 14 20%
Student > Bachelor 6 8%
Student > Master 6 8%
Student > Doctoral Student 5 7%
Other 11 15%
Unknown 13 18%
Readers by discipline Count As %
Engineering 20 28%
Chemistry 9 13%
Agricultural and Biological Sciences 6 8%
Physics and Astronomy 3 4%
Veterinary Science and Veterinary Medicine 2 3%
Other 10 14%
Unknown 21 30%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 60. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 June 2018.
All research outputs
#704,421
of 25,377,790 outputs
Outputs from Biomicrofluidics
#23
of 833 outputs
Outputs of similar age
#8,734
of 275,912 outputs
Outputs of similar age from Biomicrofluidics
#1
of 24 outputs
Altmetric has tracked 25,377,790 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 833 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.5. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 275,912 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 96% of its contemporaries.
We're also able to compare this research output to 24 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.