| Title |
A high-resolution physical map integrating an anchored chromosome with the BAC physical maps of wheat chromosome 6B
|
|---|---|
| Published in |
BMC Genomics, August 2015
|
| DOI | 10.1186/s12864-015-1803-y |
| Pubmed ID | |
| Authors |
Fuminori Kobayashi, Jianzhong Wu, Hiroyuki Kanamori, Tsuyoshi Tanaka, Satoshi Katagiri, Wataru Karasawa, Satoko Kaneko, Shota Watanabe, Toyotaka Sakaguchi, Yumiko Hanawa, Hiroko Fujisawa, Kanako Kurita, Chikako Abe, Julio C. M. Iehisa, Ryoko Ohno, Jan Šafář, Hana Šimková, Yoshiyuki Mukai, Masao Hamada, Mika Saito, Goro Ishikawa, Yuichi Katayose, Takashi R. Endo, Shigeo Takumi, Toshiki Nakamura, Kazuhiro Sato, Yasunari Ogihara, Katsuyuki Hayakawa, Jaroslav Doležel, Shuhei Nasuda, Takashi Matsumoto, Hirokazu Handa |
| Abstract |
A complete genome sequence is an essential tool for the genetic improvement of wheat. Because the wheat genome is large, highly repetitive and complex due to its allohexaploid nature, the International Wheat Genome Sequencing Consortium (IWGSC) chose a strategy that involves constructing bacterial artificial chromosome (BAC)-based physical maps of individual chromosomes and performing BAC-by-BAC sequencing. Here, we report the construction of a physical map of chromosome 6B with the goal of revealing the structural features of the third largest chromosome in wheat. We assembled 689 informative BAC contigs (hereafter reffered to as contigs) representing 91 % of the entire physical length of wheat chromosome 6B. The contigs were integrated into a radiation hybrid (RH) map of chromosome 6B, with one linkage group consisting of 448 loci with 653 markers. The order and direction of 480 contigs, corresponding to 87 % of the total length of 6B, were determined. We also characterized the contigs that contained a part of the nucleolus organizer region or centromere based on their positions on the RH map and the assembled BAC clone sequences. Analysis of the virtual gene order along 6B using the information collected for the integrated map revealed the presence of several chromosomal rearrangements, indicating evolutionary events that occurred on chromosome 6B. We constructed a reliable physical map of chromosome 6B, enabling us to analyze its genomic structure and evolutionary progression. More importantly, the physical map should provide a high-quality and map-based reference sequence that will serve as a resource for wheat chromosome 6B. |
X Demographics
The data shown below were collected from the profiles of 11 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 18% |
| Germany | 1 | 9% |
| United States | 1 | 9% |
| Japan | 1 | 9% |
| France | 1 | 9% |
| Unknown | 5 | 45% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 8 | 73% |
| Scientists | 3 | 27% |
Mendeley readers
The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 2% |
| United Kingdom | 1 | 2% |
| Unknown | 43 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 27% |
| Researcher | 12 | 27% |
| Student > Master | 4 | 9% |
| Other | 3 | 7% |
| Professor > Associate Professor | 3 | 7% |
| Other | 6 | 13% |
| Unknown | 5 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 29 | 64% |
| Biochemistry, Genetics and Molecular Biology | 6 | 13% |
| Arts and Humanities | 1 | 2% |
| Earth and Planetary Sciences | 1 | 2% |
| Engineering | 1 | 2% |
| Other | 0 | 0% |
| Unknown | 7 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 May 2016.
All research outputs
#4,517,427
of 22,821,814 outputs
Outputs from BMC Genomics
#1,869
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Outputs of similar age
#57,237
of 264,494 outputs
Outputs of similar age from BMC Genomics
#42
of 254 outputs
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