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X Demographics
Mendeley readers
Attention Score in Context
| Chapter title |
Small- and Large-Scale High Molecular Weight Genomic DNA Extraction from Planarians
|
|---|---|
| Chapter number | 7 |
| Book title |
Planarian Regeneration
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7802-1_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7800-7, 978-1-4939-7802-1
|
| Authors |
Markus A. Grohme, Miquel Vila-Farré, Jochen C. Rink, Grohme, Markus A., Vila-Farré, Miquel, Rink, Jochen C. |
| Abstract |
High-quality genomic DNA extraction is a starting point for many downstream applications in modern molecular biology. Here, we describe a simple method for isolating high molecular weight genomic DNA from planarians. The method is based on tissue lysis by a mixture of a chaotropic salt and detergent followed by organic extraction to remove proteins and lipids followed by a postpurification step to remove contaminating polysaccharides. The isolated DNA is of high molecular weight and compatible with polymerase chain reaction, cloning, or next-generation sequencing library preparation. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 18 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 3 | 17% |
| Researcher | 3 | 17% |
| Student > Ph. D. Student | 3 | 17% |
| Student > Bachelor | 2 | 11% |
| Student > Doctoral Student | 2 | 11% |
| Other | 4 | 22% |
| Unknown | 1 | 6% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 39% |
| Biochemistry, Genetics and Molecular Biology | 7 | 39% |
| Environmental Science | 1 | 6% |
| Nursing and Health Professions | 1 | 6% |
| Engineering | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 1 | 6% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 March 2019.
All research outputs
#14,417,376
of 23,090,520 outputs
Outputs from Methods in molecular biology
#4,239
of 13,207 outputs
Outputs of similar age
#240,842
of 442,634 outputs
Outputs of similar age from Methods in molecular biology
#432
of 1,499 outputs
Altmetric has tracked 23,090,520 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,207 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 64% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,634 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,499 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.