| Title |
Reduced pathological angiogenesis and tumor growth in mice lacking GPR4, a proton sensing receptor
|
|---|---|
| Published in |
Angiogenesis, November 2011
|
| DOI | 10.1007/s10456-011-9238-9 |
| Pubmed ID | |
| Authors |
Lorenza Wyder, Thomas Suply, Bérangère Ricoux, Eric Billy, Christian Schnell, Birgit U. Baumgarten, Sauveur Michel Maira, Claudia Koelbing, Mireille Ferretti, Bernd Kinzel, Matthias Müller, Klaus Seuwen, Marie-Gabrielle Ludwig |
| Abstract |
The G protein-coupled receptor GPR4 is activated by acidic pH and recent evidence indicates that it is expressed in endothelial cells. In agreement with these reports, we observe a high correlation of GPR4 mRNA expression with endothelial marker genes, and we confirm expression and acidic pH dependent function of GPR4 in primary human vascular endothelial cells. GPR4-deficient mice were generated; these are viable and fertile and show no gross abnormalities. However, these animals show a significantly reduced angiogenic response to VEGF (vascular endothelial growth factor), but not to bFGF (basic fibroblast growth factor), in a growth factor implant model. Accordingly, in two different orthotopic models, tumor growth is strongly reduced in mice lacking GPR4. Histological analysis of tumors indicates reduced tumor cell proliferation as well as altered vessel morphology, length and density. Moreover, GPR4 deficiency results in reduced VEGFR2 (VEGF Receptor 2) levels in endothelial cells, accounting, at least in part, for the observed phenotype. Our data suggest that endothelial cells sense local tissue acidosis via GPR4 and that this signal is required to generate a full angiogenic response to VEGF. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 36 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 10 | 28% |
| Student > Master | 5 | 14% |
| Student > Ph. D. Student | 4 | 11% |
| Student > Bachelor | 3 | 8% |
| Professor | 2 | 6% |
| Other | 4 | 11% |
| Unknown | 8 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 8 | 22% |
| Pharmacology, Toxicology and Pharmaceutical Science | 5 | 14% |
| Biochemistry, Genetics and Molecular Biology | 4 | 11% |
| Medicine and Dentistry | 4 | 11% |
| Neuroscience | 2 | 6% |
| Other | 3 | 8% |
| Unknown | 10 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 April 2023.
All research outputs
#15,337,507
of 23,578,918 outputs
Outputs from Angiogenesis
#351
of 548 outputs
Outputs of similar age
#95,777
of 143,464 outputs
Outputs of similar age from Angiogenesis
#2
of 3 outputs
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So far Altmetric has tracked 548 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.6. This one is in the 33rd percentile – i.e., 33% of its peers scored the same or lower than it.
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