| Title |
Direct quantification of fatty acids in wet microalgal and yeast biomass via a rapid in situ fatty acid methyl ester derivatization approach
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, August 2015
|
| DOI | 10.1007/s00253-015-6909-2 |
| Pubmed ID | |
| Authors |
Tao Dong, Liang Yu, Difeng Gao, Xiaochen Yu, Chao Miao, Yubin Zheng, Jieni Lian, Tingting Li, Shulin Chen |
| Abstract |
Accurate determination of fatty acid contents is routinely required in microalgal and yeast biofuel studies. A method of rapid in situ fatty acid methyl ester (FAME) derivatization directly from wet fresh microalgal and yeast biomass was developed in this study. This method does not require prior solvent extraction or dehydration. FAMEs were prepared with a sequential alkaline hydrolysis (15 min at 85 °C) and acidic esterification (15 min at 85 °C) process. The resulting FAMEs were extracted into n-hexane and analyzed using gas chromatography. The effects of each processing parameter (temperature, reaction time, and water content) upon the lipids quantification in the alkaline hydrolysis step were evaluated with a full factorial design. This method could tolerate water content up to 20 % (v/v) in total reaction volume, which equaled up to 1.2 mL of water in biomass slurry (with 0.05-25 mg of fatty acid). There were no significant differences in FAME quantification (p > 0.05) between the standard AOAC 991.39 method and the proposed wet in situ FAME preparation method. This fatty acid quantification method is applicable to fresh wet biomass of a wide range of microalgae and yeast species. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 2% |
| Australia | 1 | 2% |
| United Kingdom | 1 | 2% |
| Canada | 1 | 2% |
| Denmark | 1 | 2% |
| Unknown | 37 | 88% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 19% |
| Researcher | 8 | 19% |
| Student > Bachelor | 7 | 17% |
| Student > Master | 6 | 14% |
| Professor > Associate Professor | 2 | 5% |
| Other | 5 | 12% |
| Unknown | 6 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 21% |
| Chemistry | 8 | 19% |
| Chemical Engineering | 5 | 12% |
| Engineering | 4 | 10% |
| Biochemistry, Genetics and Molecular Biology | 3 | 7% |
| Other | 4 | 10% |
| Unknown | 9 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 August 2015.
All research outputs
#21,608,038
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#6,994
of 8,034 outputs
Outputs of similar age
#206,555
of 241,987 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#103
of 157 outputs
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