| Title |
Development of novel antibodies against non-structural proteins nsP1, nsP3 and nsP4 of chikungunya virus: potential use in basic research
|
|---|---|
| Published in |
Archives of Virology, August 2015
|
| DOI | 10.1007/s00705-015-2564-2 |
| Pubmed ID | |
| Authors |
Sameer Kumar, Prabhudutta Mamidi, Abhishek Kumar, Itishree Basantray, Umarani Bramha, Anshuman Dixit, Prasanta Kumar Maiti, Sujay Singh, Amol Ratnakar Suryawanshi, Subhasis Chattopadhyay, Soma Chattopadhyay |
| Abstract |
Chikungunya virus (CHIKV) has reemerged recently as an important pathogen, causing several large epidemics worldwide. This necessitates the development of better reagents to understand its biology and to establish effective and safe control measures. The present study describes the development and characterization of polyclonal antibodies (pAbs) against synthetic peptides of CHIKV non-structural proteins (nsPs; nsP1, nsP3 and nsP4). The reactivity of these pAbs was demonstrated by ELISA and Western blot. Additionally, in vitro infection studies in a mammalian system confirmed that these pAbs are highly sensitive and specific for CHIKV nsPs, as these proteins were detected very early during viral replication. Homology analysis of the selected epitope sequences revealed that they are conserved among all of the CHIKV strains of different genotypes, while comparison with other alphavirus sequences showed that none of them are 100 % identical to the epitope sequences (except Onyong-nyong and Igbo Ora viruses, which show 100 % identity to the nsP4 epitope). Interestingly, two different forms of CHIKV nsP1 and three different forms of nsP3 were detected in Western blot analysis during infection; however, further experimental investigations are required to confirm their identity. Also, the use of these antibodies demonstrated faster and enhanced expression profiles of all CHIKV nsPs in 2006 Indian outbreak strains when compared to the CHIKV prototype strain, suggesting the epidemic potential of the 2006 isolate. Accordingly, it can be suggested that the pAbs reported in this study can be used as sensitive and specific tools for experimental investigations of CHIKV replication and infection. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 61 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 2% |
| Unknown | 60 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 13 | 21% |
| Student > Master | 10 | 16% |
| Researcher | 7 | 11% |
| Student > Bachelor | 6 | 10% |
| Other | 6 | 10% |
| Other | 9 | 15% |
| Unknown | 10 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 16 | 26% |
| Agricultural and Biological Sciences | 14 | 23% |
| Immunology and Microbiology | 9 | 15% |
| Medicine and Dentistry | 3 | 5% |
| Arts and Humanities | 2 | 3% |
| Other | 5 | 8% |
| Unknown | 12 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 March 2021.
All research outputs
#7,877,230
of 24,411,829 outputs
Outputs from Archives of Virology
#950
of 4,360 outputs
Outputs of similar age
#88,414
of 270,917 outputs
Outputs of similar age from Archives of Virology
#7
of 68 outputs
Altmetric has tracked 24,411,829 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 4,360 research outputs from this source. They receive a mean Attention Score of 3.9. This one has done well, scoring higher than 77% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 270,917 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.
We're also able to compare this research output to 68 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 86% of its contemporaries.