Title |
Reversible and Selective Fluorescence Detection of Histidine Using a Naphthalimide‐Based Chemosensing Ensemble
|
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Published in |
Chemistry - An Asian Journal, August 2015
|
DOI | 10.1002/asia.201500690 |
Pubmed ID | |
Authors |
Qingtao Meng, Hongmin Jia, Xue Gao, Yue Wang, Run Zhang, Renjie Wang, Zhiqiang Zhang |
Abstract |
We described a new ensemble approach-based chemosensor, NCH-Cu2+, for highly selective and reversible detection of histidine (His) in aqueous solution and live cells. The ligand NCH exhibited specific binding with Cu2+ ions over other metal ions, accompanied with a 92.2% fluorescence quenching. The decomplexation of NCH-Cu2+ ensemble by His led to the liberation of the fluorophore, NCH, and thus the fluorescence was recovered. The specific fluorescence enhancement of NCH-Cu2+ towards His showed a good linearity with a detection of limit at 70 nM. Quantification of intracellular His at single cell level was achieved by microscopy and flow cytometry. Besides the UV-vis and emission titration, reversibility of the NCH-Cu2+ towards His was further confirmed by the imaging and cytometry analysis. In adition, microscopy studies revealed that NCH-Cu2+ was distributed in lysosome of live cells, where it could be employed as the fluorescent biosensor for imaging of His at subcellular level. |
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Geographical breakdown
Country | Count | As % |
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Unknown | 3 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 2 | 67% |
Science communicators (journalists, bloggers, editors) | 1 | 33% |
Mendeley readers
Geographical breakdown
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Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 3 | 33% |
Researcher | 2 | 22% |
Professor | 1 | 11% |
Lecturer > Senior Lecturer | 1 | 11% |
Professor > Associate Professor | 1 | 11% |
Other | 0 | 0% |
Unknown | 1 | 11% |
Readers by discipline | Count | As % |
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Chemistry | 6 | 67% |
Agricultural and Biological Sciences | 1 | 11% |
Unknown | 2 | 22% |