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Reversible and Selective Fluorescence Detection of Histidine Using a Naphthalimide‐Based Chemosensing Ensemble

Overview of attention for article published in Chemistry - An Asian Journal, August 2015
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3 X users

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Title
Reversible and Selective Fluorescence Detection of Histidine Using a Naphthalimide‐Based Chemosensing Ensemble
Published in
Chemistry - An Asian Journal, August 2015
DOI 10.1002/asia.201500690
Pubmed ID
Authors

Qingtao Meng, Hongmin Jia, Xue Gao, Yue Wang, Run Zhang, Renjie Wang, Zhiqiang Zhang

Abstract

We described a new ensemble approach-based chemosensor, NCH-Cu2+, for highly selective and reversible detection of histidine (His) in aqueous solution and live cells. The ligand NCH exhibited specific binding with Cu2+ ions over other metal ions, accompanied with a 92.2% fluorescence quenching. The decomplexation of NCH-Cu2+ ensemble by His led to the liberation of the fluorophore, NCH, and thus the fluorescence was recovered. The specific fluorescence enhancement of NCH-Cu2+ towards His showed a good linearity with a detection of limit at 70 nM. Quantification of intracellular His at single cell level was achieved by microscopy and flow cytometry. Besides the UV-vis and emission titration, reversibility of the NCH-Cu2+ towards His was further confirmed by the imaging and cytometry analysis. In adition, microscopy studies revealed that NCH-Cu2+ was distributed in lysosome of live cells, where it could be employed as the fluorescent biosensor for imaging of His at subcellular level.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 33%
Researcher 2 22%
Professor 1 11%
Lecturer > Senior Lecturer 1 11%
Professor > Associate Professor 1 11%
Other 0 0%
Unknown 1 11%
Readers by discipline Count As %
Chemistry 6 67%
Agricultural and Biological Sciences 1 11%
Unknown 2 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 December 2015.
All research outputs
#15,207,425
of 24,577,646 outputs
Outputs from Chemistry - An Asian Journal
#1,288
of 5,884 outputs
Outputs of similar age
#137,980
of 271,075 outputs
Outputs of similar age from Chemistry - An Asian Journal
#25
of 113 outputs
Altmetric has tracked 24,577,646 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,884 research outputs from this source. They receive a mean Attention Score of 1.8. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 271,075 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 113 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 76% of its contemporaries.