Title |
Autotrophic, Heterotrophic, and Mixotrophic Nitrogen Assimilation for Single-Cell Protein Production by Two Hydrogen-Oxidizing Bacterial Strains
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Published in |
Applied Biochemistry and Biotechnology, June 2018
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DOI | 10.1007/s12010-018-2824-1 |
Pubmed ID | |
Authors |
Junwei Dou, Yanmeng Huang, Haiwei Ren, Zhizhong Li, Qin Cao, Xiaofeng Liu, Dong Li |
Abstract |
To recover a nitrogen resource from high-ammonia-nitrogen wastewater, two amphitrophic hydrogen-oxidizing bacteria (HOB), Paracoccus denitrificans Y5 and P. versutus D6, capable of nitrogen assimilation for single-cell protein (SCP) production were isolated. These two HOB strains could grow autotrophically with H2 as an electron donor, O2 as an electron acceptor, CO2 as a carbon source, and ammonia nitrogen (NH4+-N) as a nitrogen source. The cell molecular formulas of strains Y5 and D6 determined by autotrophic cultivation were C3.33H6.83O2.58N0.77 and C2.87H5.34O3.17N0.57, respectively. The isolated strains could synchronously remove NH4+-N and organic carbon and produce SCP via heterotrophic cultivation. The rates of removal of NH4+-N and soluble chemical oxygen demand reached 35.47 and 49.04%, respectively, for Y5 under mixotrophic cultivation conditions with biogas slurry as a substrate. SCP content of strains Y5 and D6 was 67.34-73.73% based on cell dry weight. Compared with soybean meal, the SCP of Y5 contained a variety of amino acids. |
X Demographics
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 73 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 11 | 15% |
Student > Ph. D. Student | 9 | 12% |
Researcher | 9 | 12% |
Student > Master | 7 | 10% |
Other | 3 | 4% |
Other | 7 | 10% |
Unknown | 27 | 37% |
Readers by discipline | Count | As % |
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Engineering | 8 | 11% |
Environmental Science | 8 | 11% |
Agricultural and Biological Sciences | 7 | 10% |
Biochemistry, Genetics and Molecular Biology | 7 | 10% |
Chemical Engineering | 3 | 4% |
Other | 10 | 14% |
Unknown | 30 | 41% |