| Title |
HN-NCA heteronuclear TOCSY-NH experiment for 1HN and 15N sequential correlations in (13C, 15N) labelled intrinsically disordered proteins
|
|---|---|
| Published in |
Journal of Biomolecular NMR, August 2015
|
| DOI | 10.1007/s10858-015-9976-x |
| Pubmed ID | |
| Authors |
Christoph Wiedemann, Nishit Goradia, Sabine Häfner, Christian Herbst, Matthias Görlach, Oliver Ohlenschläger, Ramadurai Ramachandran |
| Abstract |
A simple triple resonance NMR experiment that leads to the correlation of the backbone amide resonances of each amino acid residue 'i' with that of residues 'i-1' and 'i+1' in ((13)C, (15)N) labelled intrinsically disordered proteins (IDPs) is presented. The experimental scheme, {HN-NCA heteronuclear TOCSY-NH}, exploits the favourable relaxation properties of IDPs and the presence of (1) J CαN and (2) J CαN couplings to transfer the (15)N x magnetisation from amino acid residue 'i' to adjacent residues via the application of a band-selective (15)N-(13)C(α) heteronuclear cross-polarisation sequence of ~100 ms duration. Employing non-uniform sampling in the indirect dimensions, the efficacy of the approach has been demonstrated by the acquisition of 3D HNN chemical shift correlation spectra of α-synuclein. The experimental performance of the RF pulse sequence has been compared with that of the conventional INEPT-based HN(CA)NH pulse scheme. As the availability of data from both the HCCNH and HNN experiments will make it possible to use the information extracted from one experiment to simplify the analysis of the data of the other and lead to a robust approach for unambiguous backbone and side-chain resonance assignments, a time-saving strategy for the simultaneous collection of HCCNH and HNN data is also described. |
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 20 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 35% |
| Professor | 3 | 15% |
| Student > Ph. D. Student | 3 | 15% |
| Professor > Associate Professor | 2 | 10% |
| Student > Master | 2 | 10% |
| Other | 1 | 5% |
| Unknown | 2 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 8 | 40% |
| Biochemistry, Genetics and Molecular Biology | 5 | 25% |
| Agricultural and Biological Sciences | 2 | 10% |
| Materials Science | 1 | 5% |
| Unknown | 4 | 20% |
Attention Score in Context
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#18,423,683
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Outputs of similar age from Journal of Biomolecular NMR
#7
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