| Title |
Enhanced production of carotenoids using a Thraustochytrid microalgal strain containing high levels of docosahexaenoic acid-rich oil
|
|---|---|
| Published in |
Bioprocess and Biosystems Engineering, June 2018
|
| DOI | 10.1007/s00449-018-1963-7 |
| Pubmed ID | |
| Authors |
Hansung Park, Minsoo Kwak, JeongWoo Seo, JeongHyun Ju, SunYeon Heo, SeungMoon Park, WonKyung Hong |
| Abstract |
Results to date suggest that microalgal Thraustochytrids family strains can be used to produce high-functional omega-3 rich oil (~ 30-70% of dry cell weight) and carotenoid-based antioxidant pigments simultaneously with value-added bioactive potential. In the present study, we describe the isolation and characterization of a new Thraustochytrid Schizochytrium sp. from the west coastal area of Korea. This newly isolated Thraustochytrid, identified as Schizochytrium sp. through 18S rRNA analysis and named SH104, simultaneously produces high levels of DHA and carotenoid-based antioxidant pigments. An improved Schizochytrium mutant, named SHG104, was obtained from the original host strain by γ-irradiation-induced mutagenesis. Under combined temperature-shift cultivation conditions employing white-light LEDs (light-emitting diodes), Schizochytrium sp. SHG104 yielded 10.8 g L-1 of biomass comprising 45.8% total lipids (32.1% DHA) and 4.6 mg L-1 of astaxanthin. In addition to DHA, the main fatty acids produced by Schizochytrium sp. SHG104 were palmitic acid and a trace of other long-chain fatty acids. The carotenoid profile of SH104 and SHG104 was β-carotene, astaxanthin, canthaxanthin, pheonicoxanthin and echinenone, which analyzed by HPLC and LC/APCI-MS. Furthermore, genomic analysis of Schizochytrium and Aurantiochytrium microalgae confirmed that the presence of carotenogenesis pathway enzymes and genes including geranylgeranyl diphosphate, phytoene synthase, lycopene cyclase, and cytochrome P450 hydroxylase that necessary for the production of antioxidants via a complete biosynthetic KEGG synthesis pathway. This newly isolated Schizochytrium microalga potentially have wide application as a source of antioxidants for astaxanthin-containing pigments, commercial omega-3 lipids and feed additives, such as nutritional supplements for aquaculture. |
Mendeley readers
The data shown below were compiled from readership statistics for 93 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 93 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 13 | 14% |
| Researcher | 11 | 12% |
| Student > Ph. D. Student | 10 | 11% |
| Student > Bachelor | 9 | 10% |
| Student > Doctoral Student | 4 | 4% |
| Other | 8 | 9% |
| Unknown | 38 | 41% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 16 | 17% |
| Agricultural and Biological Sciences | 16 | 17% |
| Chemical Engineering | 5 | 5% |
| Engineering | 4 | 4% |
| Immunology and Microbiology | 4 | 4% |
| Other | 9 | 10% |
| Unknown | 39 | 42% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 February 2024.
All research outputs
#8,297,754
of 25,461,852 outputs
Outputs from Bioprocess and Biosystems Engineering
#124
of 604 outputs
Outputs of similar age
#132,877
of 341,747 outputs
Outputs of similar age from Bioprocess and Biosystems Engineering
#1
of 1 outputs
Altmetric has tracked 25,461,852 research outputs across all sources so far. This one has received more attention than most of these and is in the 66th percentile.
So far Altmetric has tracked 604 research outputs from this source. They receive a mean Attention Score of 3.1. This one has done well, scoring higher than 78% of its peers.
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