Title |
Mass spectrometry identification of age-associated proteins from the malaria mosquitoes Anopheles gambiae s.s. and Anopheles stephensi
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Published in |
Data in Brief, July 2015
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DOI | 10.1016/j.dib.2015.07.007 |
Pubmed ID | |
Authors |
Maggy T. Sikulu, James Monkman, Keyur A. Dave, Marcus L. Hastie, Patricia E. Dale, Roger L. Kitching, Gerry F. Killeen, Brian H. Kay, Jeffry J. Gorman, Leon E. Hugo |
Abstract |
This study investigated proteomic changes occurring in Anopheles gambiae and Anopheles stephensi during adult mosquito aging. These changes were evaluated using two-dimensional difference gel electrophoresis (2D-DIGE) and the identities of aging related proteins were determined using capillary high-pressure liquid chromatography (capHPLC) coupled with a linear ion-trap (LTQ)-Orbitrap XL hybrid mass spectrometry (MS). Here, we have described the techniques used to determine age associated proteomic changes occurring in heads and thoraces across three age groups; 1, 9 and 17 d old A. gambiae and 4 age groups; 1, 9, 17 and 34 d old A. stephensi. We have provided normalised spot volume raw data for all protein spots that were visible on 2D-DIGE images for both species and processed Orbitrap mass spectrometry data. For public access, mass spectrometry raw data are available via ProteomeXchange with identifier PXD002153. A detailed description of this study has been described elsewhere [1]. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
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Madagascar | 1 | 3% |
Sudan | 1 | 3% |
Unknown | 35 | 95% |
Demographic breakdown
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Student > Ph. D. Student | 8 | 22% |
Researcher | 6 | 16% |
Student > Master | 4 | 11% |
Other | 2 | 5% |
Professor | 2 | 5% |
Other | 9 | 24% |
Unknown | 6 | 16% |
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Biochemistry, Genetics and Molecular Biology | 5 | 14% |
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Computer Science | 1 | 3% |
Immunology and Microbiology | 1 | 3% |
Other | 8 | 22% |
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