| Title |
Comprehensive evaluation of methods to isolate, quantify, and characterize circulating cell-free DNA from small volumes of plasma
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, June 2015
|
| DOI | 10.1007/s00216-015-8846-4 |
| Pubmed ID | |
| Authors |
Florence Mauger, Cécile Dulary, Christian Daviaud, Jean-François Deleuze, Jorg Tost |
| Abstract |
Circulating cell-free DNA (ccfDNA) has great potential for non-invasive diagnostics, and prediction and monitoring of treatment response, but its amount is usually limited. Therefore, the choice of methods to extract and characterize ccfDNA is crucial. In the current study, we performed the most comprehensive comparison of methods for ccfDNA extraction (11 methods), quantification (3 methods), and estimation of the integrity index (2 methods) from small quantities of different kinds of plasma. The QIAamp® Circulating Nucleic Acid Kit and the Norgen Plasma/Serum Circulating DNA Purification Mini Kit showed the best accuracy and reproducibility, but the Norgen kit allowed to extract a higher amount of ccfDNA. This workflow provides a reliable protocol for the multiple applications of ccfDNA in biomedicine. Graphical Abstract Workflow for the evaluation of methods to isolate, quantify and characterize circulating cell-free DNA from small volumes of plasma. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 67% |
| Members of the public | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 147 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Ireland | 1 | <1% |
| Romania | 1 | <1% |
| Unknown | 145 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 35 | 24% |
| Researcher | 29 | 20% |
| Student > Master | 20 | 14% |
| Other | 11 | 7% |
| Student > Bachelor | 7 | 5% |
| Other | 17 | 12% |
| Unknown | 28 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 50 | 34% |
| Medicine and Dentistry | 17 | 12% |
| Agricultural and Biological Sciences | 15 | 10% |
| Engineering | 8 | 5% |
| Chemical Engineering | 3 | 2% |
| Other | 15 | 10% |
| Unknown | 39 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 April 2016.
All research outputs
#16,721,717
of 25,374,647 outputs
Outputs from Analytical & Bioanalytical Chemistry
#5,259
of 9,619 outputs
Outputs of similar age
#156,116
of 277,856 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#44
of 178 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 9,619 research outputs from this source. They receive a mean Attention Score of 3.1. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
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We're also able to compare this research output to 178 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.