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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Rapid concentration and sensitive detection of hookworm ova from wastewater matrices using a real-time PCR method
|
|---|---|
| Published in |
Experimental Parasitology, August 2015
|
| DOI | 10.1016/j.exppara.2015.08.009 |
| Pubmed ID | |
| Authors |
P. Gyawali, J.P.S. Sidhu, W. Ahmed, P. Jagals, S. Toze |
| Abstract |
The risk of human hookworm infections from land application of wastewater matrices could be high in regions with high hookworm prevalence. A rapid, sensitive and specific hookworm detection method from wastewater matrices is required in order to assess human health risks. Currently available methods used to identify hookworm ova to the species level are time consuming and lack accuracy. In this study, a real-time PCR method was developed for the rapid, sensitive and specific detection of canine hookworm (Ancylostoma caninum) ova from wastewater matrices. A. caninum was chosen because of its morphological similarity to the human hookworm (Ancylostoma duodenale and Necator americanus). The newly developed PCR method has low detection sensitivity with the ability to detect less than one A. caninum ova from 1 L of secondary treated wastewater at the mean threshold cycle (CT) values ranging from 30.1-34.3. The method is also able to detect four A. caninum ova from 1 L of raw wastewater and from ∼ 4 gm of treated sludge with mean CT values ranging from 35.6-39.8 and 39.8-39.9, respectively. The better detection sensitivity obtained for secondary treated wastewater compared to raw wastewater and sludge samples could be attributed to sample turbidity. The proposed method appears to be rapid, sensitive and specific compared to traditional methods and has potential to aid in the public health risk assessment associated with land application of wastewater matrices. Furthermore, the method can be adapted to detect other helminth ova of interest from wastewater matrices. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Malaysia | 1 | 3% |
| Unknown | 32 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 10 | 30% |
| Student > Ph. D. Student | 6 | 18% |
| Student > Doctoral Student | 3 | 9% |
| Student > Bachelor | 3 | 9% |
| Student > Master | 2 | 6% |
| Other | 1 | 3% |
| Unknown | 8 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 27% |
| Environmental Science | 3 | 9% |
| Immunology and Microbiology | 3 | 9% |
| Biochemistry, Genetics and Molecular Biology | 2 | 6% |
| Medicine and Dentistry | 2 | 6% |
| Other | 4 | 12% |
| Unknown | 10 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 August 2015.
All research outputs
#20,657,128
of 25,374,917 outputs
Outputs from Experimental Parasitology
#1,371
of 1,826 outputs
Outputs of similar age
#203,705
of 277,645 outputs
Outputs of similar age from Experimental Parasitology
#16
of 35 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,826 research outputs from this source. They receive a mean Attention Score of 2.9. This one is in the 13th percentile – i.e., 13% of its peers scored the same or lower than it.
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We're also able to compare this research output to 35 others from the same source and published within six weeks on either side of this one. This one is in the 34th percentile – i.e., 34% of its contemporaries scored the same or lower than it.