You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
X Demographics
Mendeley readers
Attention Score in Context
| Title |
Self-Assembled Functional Nanostructure of Plasmid DNA with Ionic Liquid [Bmim][PF6]: Enhanced Efficiency in Bacterial Gene Transformation
|
|---|---|
| Published in |
Langmuir, April 2015
|
| DOI | 10.1021/acs.langmuir.5b00402 |
| Pubmed ID | |
| Authors |
Sarvesh K. Soni, Sampa Sarkar, Nedaossadat Mirzadeh, P. R. Selvakannan, Suresh K. Bhargava |
| Abstract |
The electrostatic interaction between the negatively charged phosphate groups of plasmid DNA and the cationic part of hydrophobic Ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate ([Bmim][PF6]), initiate the spontaneous self-assembly to form the functional nanostructures made up of DNA and ionic liquid (IL). These functional nanostructures were demonstrated as promising synthetic non-viral vectors for the efficient bacterial pGFP gene transformation in cells. In particular, the functional nanostructures that were made up of 1µL of IL ([Bmim][PF6]) and 1 µg plasmid DNA can increase the transformation efficiency by 300% - 400% in microbial system, without showing any toxicity on E.Coli DH5α cells. (31)P Nuclear Magnetic Resonance (NMR), Fourier Transform Infrared (FT-IR) spectroscopy and X-ray Photoelectron (XPS) spectroscopic analysis revealed that the electrostatic interaction between negatively charged phosphate oxygen and cationic Bmim(+) tend to initiate the self-assembly process. Thermogravimetric analysis of the DNA-IL functional nanostructures showed that these nanostructures consist of ~16 weight % of Ionic liquid, which is considered to provide the stability to the plasmid DNA that eventually enhanced the transformation efficiency. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Iran, Islamic Republic of | 1 | 2% |
| Unknown | 41 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 15 | 36% |
| Researcher | 9 | 21% |
| Student > Master | 6 | 14% |
| Other | 3 | 7% |
| Professor > Associate Professor | 2 | 5% |
| Other | 3 | 7% |
| Unknown | 4 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 22 | 52% |
| Biochemistry, Genetics and Molecular Biology | 7 | 17% |
| Chemical Engineering | 2 | 5% |
| Materials Science | 2 | 5% |
| Immunology and Microbiology | 1 | 2% |
| Other | 1 | 2% |
| Unknown | 7 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 March 2021.
All research outputs
#14,824,070
of 22,826,360 outputs
Outputs from Langmuir
#10,318
of 13,954 outputs
Outputs of similar age
#148,209
of 263,976 outputs
Outputs of similar age from Langmuir
#52
of 106 outputs
Altmetric has tracked 22,826,360 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,954 research outputs from this source. They receive a mean Attention Score of 3.8. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 263,976 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 106 others from the same source and published within six weeks on either side of this one. This one is in the 49th percentile – i.e., 49% of its contemporaries scored the same or lower than it.