You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
X Demographics
Mendeley readers
Attention Score in Context
| Title |
Engineering circular RNA for potent and stable translation in eukaryotic cells
|
|---|---|
| Published in |
Nature Communications, July 2018
|
| DOI | 10.1038/s41467-018-05096-6 |
| Pubmed ID | |
| Authors |
R. Alexander Wesselhoeft, Piotr S. Kowalski, Daniel G. Anderson |
| Abstract |
Messenger RNA (mRNA) has broad potential for application in biological systems. However, one fundamental limitation to its use is its relatively short half-life in biological systems. Here we develop exogenous circular RNA (circRNA) to extend the duration of protein expression from full-length RNA messages. First, we engineer a self-splicing intron to efficiently circularize a wide range of RNAs up to 5 kb in length in vitro by rationally designing ubiquitous accessory sequences that aid in splicing. We maximize translation of functional protein from these circRNAs in eukaryotic cells, and we find that engineered circRNA purified by high performance liquid chromatography displays exceptional protein production qualities in terms of both quantity of protein produced and stability of production. This study pioneers the use of exogenous circRNA for robust and stable protein expression in eukaryotic cells and demonstrates that circRNA is a promising alternative to linear mRNA. |
X Demographics
The data shown below were collected from the profiles of 14 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 6 | 43% |
| Netherlands | 2 | 14% |
| United Kingdom | 1 | 7% |
| France | 1 | 7% |
| Unknown | 4 | 29% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 8 | 57% |
| Scientists | 3 | 21% |
| Science communicators (journalists, bloggers, editors) | 2 | 14% |
| Practitioners (doctors, other healthcare professionals) | 1 | 7% |
Mendeley readers
The data shown below were compiled from readership statistics for 633 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 633 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 123 | 19% |
| Researcher | 94 | 15% |
| Student > Bachelor | 47 | 7% |
| Student > Master | 43 | 7% |
| Other | 30 | 5% |
| Other | 72 | 11% |
| Unknown | 224 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 200 | 32% |
| Agricultural and Biological Sciences | 74 | 12% |
| Chemistry | 22 | 3% |
| Immunology and Microbiology | 19 | 3% |
| Medicine and Dentistry | 18 | 3% |
| Other | 70 | 11% |
| Unknown | 230 | 36% |
Attention Score in Context
This research output has an Altmetric Attention Score of 62. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 April 2024.
All research outputs
#698,172
of 25,707,225 outputs
Outputs from Nature Communications
#12,020
of 58,159 outputs
Outputs of similar age
#14,904
of 342,006 outputs
Outputs of similar age from Nature Communications
#308
of 1,269 outputs
Altmetric has tracked 25,707,225 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 58,159 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 55.5. This one has done well, scoring higher than 79% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 342,006 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 95% of its contemporaries.
We're also able to compare this research output to 1,269 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 75% of its contemporaries.